دورية أكاديمية
Immunocytochemistry of matrix proteins in calcified tissues: functional biochemistry on section.
| العنوان: | Immunocytochemistry of matrix proteins in calcified tissues: functional biochemistry on section. |
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| المؤلفون: | Nanci A; Laboratory for the Study of Calcified Tissues and Biomaterials, Faculty of Dentistry, Université of Montréal, Montréal, Canada. antonio.nanci@umontreal.ca, Wazen R, Nishio C, Zalzal SF |
| المصدر: | European journal of histochemistry : EJH [Eur J Histochem] 2008 Oct-Dec; Vol. 52 (4), pp. 201-14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: PagePress Publications Country of Publication: Italy NLM ID: 9207930 Publication Model: Print Cited Medium: Print ISSN: 1121-760X (Print) Linking ISSN: 1121760X NLM ISO Abbreviation: Eur J Histochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2010- : Pavia : PagePress Publications Original Publication: Pavia : Luigi Ponzio, [1992- |
| مواضيع طبية MeSH: | Bone and Bones/*metabolism , Calcinosis/*metabolism , Extracellular Matrix Proteins/*metabolism , Immunohistochemistry/*methods, Ameloblasts/chemistry ; Ameloblasts/ultrastructure ; Animals ; Bone and Bones/ultrastructure ; Gold Colloid ; Humans ; Microscopy, Electron, Transmission ; Models, Animal |
| مستخلص: | The organic matrix of calcified tissues comprises collagenous and/or noncollagenous matrix proteins (NCPs). Identification and precise mapping of these matrix components is essential for determining their function, formulating coherent hypotheses on their mechanism(s) of action, and developing novel therapeutic approaches based on biologics. Fibrillar collagen can be readily identified by its conspicuous structure, however, NCPs, in general, do not individually exhibit characteristic structural features that permit to identify them and morphologically determine their localization. To address this limitation, we have used immunocytochemistry, a form of "biochemistry on section", to correlate composition with structure. For cytochemical characterizations, including immunolabeling, our laboratory has opted for colloidal gold labelings and pioneered their application to calcified tissues because they yield high spatial resolution and are quantitative. Over the years, this approach has been applied to identify and map various NCPs in bone and teeth and, in this review of our work, we will emphasize some selected studies that highlight it application to also achieve functional information. |
| Number of References: | 77 |
| المشرفين على المادة: | 0 (Extracellular Matrix Proteins) 0 (Gold Colloid) 117924-90-0 (Aurodye) |
| تواريخ الأحداث: | Date Created: 20081226 Date Completed: 20090212 Latest Revision: 20220331 |
| رمز التحديث: | 20240628 |
| DOI: | 10.4081/1218 |
| PMID: | 19109094 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1121-760X |
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| DOI: | 10.4081/1218 |