دورية أكاديمية
Determination of zinc using carbonic anhydrase-based fluorescence biosensors.
| العنوان: | Determination of zinc using carbonic anhydrase-based fluorescence biosensors. |
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| المؤلفون: | Bozym R; Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA., Hurst TK, Westerberg N, Stoddard A, Fierke CA, Frederickson CJ, Thompson RB |
| المصدر: | Methods in enzymology [Methods Enzymol] 2008; Vol. 450, pp. 287-309. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Biosensing Techniques*, Carbonic Anhydrases/*chemistry , Spectrometry, Fluorescence/*methods , Zinc/*analysis |
| مستخلص: | This chapter summarizes the use of carbonic anhydrase (CA)-based fluorescent indicators to determine free zinc in solution, in cells, and in subcellular organelles. Expression (both in situ and in vitro) and preparation of CA-based indicators are described, together with techniques of their use, and procedures to minimize contamination. Recipes for zinc buffers are supplied. |
| معلومات مُعتمدة: | T32 GM145304 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | EC 4.2.1.1 (Carbonic Anhydrases) J41CSQ7QDS (Zinc) |
| تواريخ الأحداث: | Date Created: 20090121 Date Completed: 20090302 Latest Revision: 20231023 |
| رمز التحديث: | 20231023 |
| DOI: | 10.1016/S0076-6879(08)03414-9 |
| PMID: | 19152866 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1557-7988 |
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| DOI: | 10.1016/S0076-6879(08)03414-9 |