دورية أكاديمية
The acute-phase protein serum amyloid A3 is expressed in the bovine mammary gland and plays a role in host defence.
| العنوان: | The acute-phase protein serum amyloid A3 is expressed in the bovine mammary gland and plays a role in host defence. |
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| المؤلفون: | Molenaar AJ; Dairy Science and Technology, Ruakura Research Centre, Hamilton, New Zealand. adrian.molenaar@agresearch.co.nz, Harris DP, Rajan GH, Pearson ML, Callaghan MR, Sommer L, Farr VC, Oden KE, Miles MC, Petrova RS, Good LL, Singh K, McLaren RD, Prosser CG, Kim KS, Wieliczko RJ, Dines MH, Johannessen KM, Grigor MR, Davis SR, Stelwagen K |
| المصدر: | Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals [Biomarkers] 2009 Feb; Vol. 14 (1), pp. 26-37. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Tayor & Francis Country of Publication: England NLM ID: 9606000 Publication Model: Print Cited Medium: Internet ISSN: 1366-5804 (Electronic) Linking ISSN: 1354750X NLM ISO Abbreviation: Biomarkers Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Abingdon, Oxford : Tayor & Francis Original Publication: London : Taylor & Francis, c1996- |
| مواضيع طبية MeSH: | Mammary Glands, Animal/*metabolism , Serum Amyloid A Protein/*metabolism, Animals ; Base Sequence ; Blotting, Northern ; Blotting, Western ; Cattle ; DNA Primers ; Enzyme-Linked Immunosorbent Assay ; Polymerase Chain Reaction ; RNA, Messenger/genetics ; Serum Amyloid A Protein/genetics ; Serum Amyloid A Protein/immunology |
| مستخلص: | The serum amyloid A protein is one of the major reactants in the acute-phase response. Using representational difference analysis comparing RNA from normal and involuting quarters of a dairy cow mammary gland, we found an mRNA encoding the SAA3 protein (M-SAA3). The M-SAA3 mRNA was localized to restricted populations of bovine mammary epithelial cells (MECs). It was expressed at a moderate level in late pregnancy, at a low level through lactation, was induced early in milk stasis, and expressed at high levels in most MECs during mid to late involution and inflammation/mastitis. The mature M-SAA3 peptide was expressed in Escherichia coli, antibodies made, and shown to have antibacterial activity against E. coli, Streptococcus uberis and Pseudomonas aeruginosa. These results suggest that the mammary SAA3 may have a role in protection of the mammary gland during remodelling and infection and possibly in the neonate gastrointestinal tract. |
| المشرفين على المادة: | 0 (DNA Primers) 0 (RNA, Messenger) 0 (Serum Amyloid A Protein) |
| تواريخ الأحداث: | Date Created: 20090314 Date Completed: 20090528 Latest Revision: 20220330 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1080/13547500902730714 |
| PMID: | 19283521 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1366-5804 |
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| DOI: | 10.1080/13547500902730714 |