دورية أكاديمية
أعرض في EDS

Reduced expression of IL-3 mediates intestinal mast cell depletion in diabetic rats: role of insulin and glucocorticoid hormones.

التفاصيل البيبلوغرافية
العنوان: Reduced expression of IL-3 mediates intestinal mast cell depletion in diabetic rats: role of insulin and glucocorticoid hormones.
المؤلفون: Carvalho Vde F; Laboratório de Inflamação, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Manguinhos, Rio de Janeiro, RJ, Brazil., Barreto Ede O, Farias-Filho FA, Gomes LH, Mendonça Lde L, Cordeiro RS, Martins MA, Rodrigues e Silva PM
المصدر: International journal of experimental pathology [Int J Exp Pathol] 2009 Apr; Vol. 90 (2), pp. 148-55.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: England NLM ID: 9014042 Publication Model: Print Cited Medium: Internet ISSN: 1365-2613 (Electronic) Linking ISSN: 09599673 NLM ISO Abbreviation: Int J Exp Pathol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, OX : Wiley, c1990-
مواضيع طبية MeSH: Diabetes Mellitus, Experimental/*pathology , Ileum/*pathology , Interleukin-3/*metabolism , Mast Cells/*pathology, Animals ; Cell Count ; Diabetes Mellitus, Experimental/metabolism ; Down-Regulation/drug effects ; Glucocorticoids/antagonists & inhibitors ; Glucocorticoids/physiology ; Hormone Antagonists/pharmacology ; Ileum/drug effects ; Ileum/metabolism ; Insulin/pharmacology ; Interleukin-3/genetics ; Male ; Mast Cells/drug effects ; Mast Cells/metabolism ; Mifepristone/pharmacology ; RNA, Messenger/genetics ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction/methods
مستخلص: Rats turned diabetic by alloxan treatment are refractory to systemic anaphylactic shock, in a direct association with reduced intestinal haemorrhage and tissue response to antigen challenge. As diabetic rats show reduction in mast cell numbers in different body compartments, this study was undertaken to investigate the influence of alloxan diabetes on mast cell population as well as the expression of the mast cell growth factor interleukin (IL)-3 in the small intestine of rats. We also analysed the putative involvement of endogenous insulin and glucocorticoid hormones in this phenomenon. There was a significant decrease in the number of mast cells present in the small intestine (ileum segment) of diabetic rats. Likewise, the immunohistochemical analysis revealed that IL-3 labelling was markedly attenuated in diabetic rats, as compared with normal animals, a phenomenon which paralleled with a decreased mRNA expression as attested by Reverse transcriptase-polymerase chain reaction technique. Treatment with insulin and with the steroid receptor antagonist RU 486 restored basal mast cell numbers, normal levels of IL-3 labelling and mRNA expression for IL-3 in the ileum of diabetic rats. In conclusion, our findings show that there is a causative relationship between down-regulation of mast cell numbers and the expression of IL-3 associated with diabetic state. In addition, as both parameters were suppressed by administration of insulin and RU 486, it indicates that an imbalance between the systemic levels of insulin and glucocorticoid hormones seems to be implicated in the reduction in intestinal mast cell population and refractoriness to antigen provocation in alloxan diabetes.
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المشرفين على المادة: 0 (Glucocorticoids)
0 (Hormone Antagonists)
0 (Insulin)
0 (Interleukin-3)
0 (RNA, Messenger)
320T6RNW1F (Mifepristone)
تواريخ الأحداث: Date Created: 20090402 Date Completed: 20090514 Latest Revision: 20211020
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC2676696
DOI: 10.1111/j.1365-2613.2008.00620.x
PMID: 19335553
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2613
DOI:10.1111/j.1365-2613.2008.00620.x