دورية أكاديمية
أعرض في EDS

A mouse model expressing a truncated form of ameloblastin exhibits dental and junctional epithelium defects.

التفاصيل البيبلوغرافية
العنوان: A mouse model expressing a truncated form of ameloblastin exhibits dental and junctional epithelium defects.
المؤلفون: Wazen RM; Laboratory for the Study of Calcified Tissues and Biomaterials, Faculty of Dentistry, Université de Montréal, Station Centre-Ville, Montreal, Quebec, Canada., Moffatt P, Zalzal SF, Yamada Y, Nanci A
المصدر: Matrix biology : journal of the International Society for Matrix Biology [Matrix Biol] 2009 Jun; Vol. 28 (5), pp. 292-303. Date of Electronic Publication: 2009 Apr 16.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9432592 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1569-1802 (Electronic) Linking ISSN: 0945053X NLM ISO Abbreviation: Matrix Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Stuttgart ; New York : Fischer, c1994-
مواضيع طبية MeSH: Dental Enamel Proteins*/genetics , Dental Enamel Proteins*/metabolism , Epithelial Cells*/metabolism , Epithelial Cells*/pathology , Incisor*/physiology , Incisor*/ultrastructure , Tooth Abnormalities*/genetics , Tooth Abnormalities*/metabolism , Tooth Abnormalities*/pathology, Ameloblasts/cytology ; Ameloblasts/metabolism ; Amino Acid Sequence ; Animals ; Biomarkers/metabolism ; Enamel Organ/metabolism ; Extracellular Matrix/metabolism ; Female ; Genotype ; Male ; Mice ; Mice, Knockout ; Mice, Transgenic ; Molecular Sequence Data
مستخلص: Ameloblastin (AMBN) is the second most abundant extracellular matrix protein produced by the epithelial cells called ameloblasts and is found mainly in forming dental enamel. Inactivation of its expression by gene knockout results in absence of the enamel layer and its replacement by a thin layer of dysplastic mineralized matrix. The objective of this study was to further characterize the enamel organ and mineralized matrix produced in the AMBN knockout mouse. However, in the course of our study, we unexpectedly found that this mouse is in fact a mutant that does not express the full-length protein but that produces a truncated form of AMBN. Mandibles from wild type and mutant mice were processed for morphological analyses and immunolabeling. Microdissected enamel organs and associated matrix were also prepared for molecular and biochemical analyses. In incisors from mutants, ameloblasts lost their polarized organization and the enamel organ detached from the tooth surface and became disorganized. A thin layer of dysplastic mineralized material was deposited onto dentin, and mineralized masses were present within the enamel organ. These mineralized materials generated lower backscattered electron contrast than normal enamel, and immunocytochemistry with colloidal gold revealed the presence of amelogenin, bone sialoprotein and osteopontin. In addition, the height of the alveolar bone was reduced, and the junctional epithelium lost its integrity. Immunochemical and RT-PCR results revealed that the altered enamel organ in the mutant mice produced a shorter AMBN protein that is translated from truncated RNA missing exons 5 and 6. These results indicate that absence of full-length protein and/or expression of an incomplete protein have direct/indirect effects beyond structuring of mineral during enamel formation, and highlight potential functional regions on the AMBN molecule.
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معلومات مُعتمدة: 89811-1 Canada CAPMC CIHR; Z01 DE000720 United States ImNIH Intramural NIH HHS; Z01 DE000720-01 United States ImNIH Intramural NIH HHS; DE 000720-02 United States DE NIDCR NIH HHS
المشرفين على المادة: 0 (Ambn protein, mouse)
0 (Biomarkers)
0 (Dental Enamel Proteins)
تواريخ الأحداث: Date Created: 20090421 Date Completed: 20090914 Latest Revision: 20220224
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC2727877
DOI: 10.1016/j.matbio.2009.04.004
PMID: 19375505
قاعدة البيانات: MEDLINE
الوصف
تدمد:1569-1802
DOI:10.1016/j.matbio.2009.04.004