دورية أكاديمية
CO2 activates orexin-containing neurons in mice.
| العنوان: | CO2 activates orexin-containing neurons in mice. |
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| المؤلفون: | Sunanaga J; Department of Physiology, Kagoshima University Graduate School of Medical & Dental Sciences, Kagoshima 890-8544, Japan. jinko@m2.kufm.kagoshima-u.ac.jp, Deng BS, Zhang W, Kanmura Y, Kuwaki T |
| المصدر: | Respiratory physiology & neurobiology [Respir Physiol Neurobiol] 2009 May 15; Vol. 166 (3), pp. 184-6. Date of Electronic Publication: 2009 Mar 24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101140022 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1519 (Electronic) Linking ISSN: 15699048 NLM ISO Abbreviation: Respir Physiol Neurobiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2002- |
| مواضيع طبية MeSH: | Brain/*cytology , Carbon Dioxide/*pharmacology , Intracellular Signaling Peptides and Proteins/*metabolism , Neurons/*drug effects , Neuropeptides/*metabolism, Animals ; Brain/drug effects ; Cell Count ; Male ; Mice ; Mice, Inbred C57BL ; Neurons/cytology ; Orexins ; Proto-Oncogene Proteins c-fos/metabolism |
| مستخلص: | Accumulating evidence shows possible participation of orexin, a hypothalamic neuropeptide also known as hypocretin, in regulation of breathing. In this study we examined whether hypercapnic stimulation in vivo activates orexin-containing neurons in the hypothalamus. A hypercapnic gas mixture of 10% CO(2), 21% O(2), and residual N(2) or room air was inhaled to awake and freely moving mice for 3h. Their brains were examined by double immunohistochemical staining for orexin and c-Fos. We found that inhalation of CO(2) significantly increased c-Fos expression in the orexin-containing cells that were located in the perifornical region and the dorsomedial hypothalamus (15.8+/-1.6% for room air vs. 28.4+/-2.7% for CO(2), p=0.011) but not in the lateral hypothalamic area (12.3+/-1.2% for room air vs. 12.4+/-3.0% for CO(2)). Although current methodology cannot differentiate between direct effect of CO(2) on the orexin-containing neurons and indirect one through other neurons, this is the first report showing that inhalation of CO(2) did activate the orexin-containing neurons in vivo. |
| المشرفين على المادة: | 0 (Intracellular Signaling Peptides and Proteins) 0 (Neuropeptides) 0 (Orexins) 0 (Proto-Oncogene Proteins c-fos) 142M471B3J (Carbon Dioxide) |
| تواريخ الأحداث: | Date Created: 20090516 Date Completed: 20090825 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.resp.2009.03.006 |
| PMID: | 19442935 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-1519 |
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| DOI: | 10.1016/j.resp.2009.03.006 |