دورية أكاديمية
High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class I ligands using real-time PCR.
| العنوان: | High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class I ligands using real-time PCR. |
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| المؤلفون: | Koehler RN; Division of Retrovirology, US Military HIV Research Program/Henry M. Jackson Foundation, Rockville, MD 20850, USA. rkoehler@hivresearch.org, Walsh AM, Moqueet N, Currier JR, Eller MA, Eller LA, Wabwire-Mangen F, Michael NL, Robb ML, McCutchan FE, Kijak GH |
| المصدر: | Tissue antigens [Tissue Antigens] 2009 Jul; Vol. 74 (1), pp. 73-80. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Blackwell Country of Publication: England NLM ID: 0331072 Publication Model: Print Cited Medium: Internet ISSN: 1399-0039 (Electronic) Linking ISSN: 00012815 NLM ISO Abbreviation: Tissue Antigens Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2015>: [Oxford] : Wiley Blackwell Original Publication: 1971- : Copenhagen, Munksgaard. |
| مواضيع طبية MeSH: | Histocompatibility Antigens Class I/*genetics , Polymerase Chain Reaction/*methods , Receptors, KIR2DL2/*genetics , Receptors, KIR2DL3/*genetics , Receptors, KIR3DL1/*genetics , Receptors, KIR3DS1/*genetics, Alleles ; Genotype ; Humans ; Ligands ; Sensitivity and Specificity |
| مستخلص: | Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies. |
| معلومات مُعتمدة: | Y1-A1-2642-11 United States PHS HHS |
| المشرفين على المادة: | 0 (Histocompatibility Antigens Class I) 0 (KIR2DL2 protein, human) 0 (KIR2DL3 protein, human) 0 (KIR3DL1 protein, human) 0 (Ligands) 0 (Receptors, KIR2DL2) 0 (Receptors, KIR2DL3) 0 (Receptors, KIR3DL1) 0 (Receptors, KIR3DS1) |
| تواريخ الأحداث: | Date Created: 20090616 Date Completed: 20090821 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/j.1399-0039.2009.01265.x |
| PMID: | 19522772 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1399-0039 |
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| DOI: | 10.1111/j.1399-0039.2009.01265.x |