التفاصيل البيبلوغرافية
| العنوان: |
Detection of Laem-Singh virus in cultured Penaeus monodon shrimp from several sites in the Indo-Pacific region. |
| المؤلفون: |
Sittidilokratna N; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Phathumthani 12120, Thailand., Dangtip S, Sritunyalucksana K, Babu R, Pradeep B, Mohan CV, Gudkovs N, Walker PJ |
| المصدر: |
Diseases of aquatic organisms [Dis Aquat Organ] 2009 Apr 27; Vol. 84 (3), pp. 195-200. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Inter-Research Country of Publication: Germany NLM ID: 8807037 Publication Model: Print Cited Medium: Print ISSN: 0177-5103 (Print) Linking ISSN: 01775103 NLM ISO Abbreviation: Dis Aquat Organ Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Oldendorf/Luhe, Federal Republic of Germany : Inter-Research, c1985- |
| مواضيع طبية MeSH: |
Penaeidae/*virology , RNA Viruses/*genetics , RNA Viruses/*isolation & purification, Animals ; Indian Ocean ; Pacific Ocean ; Phylogeny ; RNA Viruses/classification |
| مستخلص: |
Laem-Singh virus (LSNV) is a positive-sense single-stranded RNA (ssRNA) virus that was recently identified in Penaeus monodon shrimp in Thailand displaying signs of slow growth syndrome. A total of 326 shrimp collected between 1998 and 2007 from countries in the Indo-Pacific region were tested by RT-PCR for evidence of LSNV infection. The samples comprised batches of whole postlarvae, and lymphoid organ, gill, muscle or pleopod tissue of juvenile, subadult and adult shrimp. LSNV was not detected in 96 P. monodon, P. japonicus or P. merguiensis from Australia or 16 P. monodon from Fiji, Philippines, Sri Lanka and Mozambique. There was no evidence of LSNV infection in 73 healthy juvenile P. vannamei collected during 2006 from ponds at 9 locations in Thailand. However, LNSV was detected in each of 6 healthy P. monodon tested from Malaysia and Indonesia, 2 of 6 healthy P. monodon tested from Vietnam and 39 of 40 P. monodon collected from slow-growth ponds in Thailand. A survey of 81 P. monodon collected in 2007 from Andhra Pradesh, India, indicated 56.8% prevalence of LSNV infection but no clear association with disease or slow growth. Phylogenetic analysis of PCR amplicons obtained from samples from India, Vietnam, Malaysia and Thailand indicated that nucleotide sequence variation was very low (>98% identity) and there was no clustering of viruses according to site of isolation or the health status of the shrimp. The data suggests that LSNV exists as a single genetic lineage and occurs commonly in healthy P. monodon in parts of Asia. |
| تواريخ الأحداث: |
Date Created: 20090702 Date Completed: 20090811 Latest Revision: 20090701 |
| رمز التحديث: |
20240829 |
| DOI: |
10.3354/dao02059 |
| PMID: |
19565696 |
| قاعدة البيانات: |
MEDLINE |
