دورية أكاديمية
[Preparation and characterization of monoclonal antibodies to the cholera toxin].
| العنوان: | [Preparation and characterization of monoclonal antibodies to the cholera toxin]. |
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| المؤلفون: | Petrova EE; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho-Maklaya 16/10, V-437, Moscow, 117997 Russia., Komaleva RL, Lakhtina OE, Samokhvalova LV, Kalinina NA, Shoshina NS, Rubina AIu, Filippova MA, Vertiev IuV, Valyakina TI, Grishin EV |
| المصدر: | Bioorganicheskaia khimiia [Bioorg Khim] 2009 May-Jun; Vol. 35 (3), pp. 357-67. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Nauka Country of Publication: Russia (Federation) NLM ID: 7804941 Publication Model: Print Cited Medium: Print ISSN: 0132-3423 (Print) Linking ISSN: 01323423 NLM ISO Abbreviation: Bioorg Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva, Nauka |
| مواضيع طبية MeSH: | Antibodies, Bacterial/*immunology , Antibodies, Monoclonal/*immunology , Cholera Toxin/*immunology, Antibodies, Monoclonal/isolation & purification ; Bacterial Toxins/immunology ; Cross Reactions ; Escherichia coli Proteins/immunology ; Immunoenzyme Techniques ; Microarray Analysis |
| مستخلص: | Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA. |
| المشرفين على المادة: | 0 (Antibodies, Bacterial) 0 (Antibodies, Monoclonal) 0 (Bacterial Toxins) 0 (Escherichia coli Proteins) 9012-63-9 (Cholera Toxin) |
| تواريخ الأحداث: | Date Created: 20090722 Date Completed: 20090811 Latest Revision: 20191027 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1134/s1068162009030078 |
| PMID: | 19621051 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0132-3423 |
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| DOI: | 10.1134/s1068162009030078 |