دورية أكاديمية
Alternative splicing generates a novel FADS2 alternative transcript in baboons.
| العنوان: | Alternative splicing generates a novel FADS2 alternative transcript in baboons. |
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| المؤلفون: | Park WJ; Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA., Reardon HT, Tyburczy C, Kothapalli KS, Brenna JT |
| المصدر: | Molecular biology reports [Mol Biol Rep] 2010 Jun; Vol. 37 (5), pp. 2403-6. Date of Electronic Publication: 2009 Aug 20. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dordrecht, Boston, Reidel. |
| مواضيع طبية MeSH: | Alternative Splicing/*genetics , Fatty Acid Desaturases/*genetics , Papio/*genetics, Animals ; Cell Line, Tumor ; Gene Expression Profiling ; Gene Expression Regulation, Enzymologic ; Humans ; Protein Transport ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Subcellular Fractions/enzymology |
| مستخلص: | The mammalian fatty acid desaturase 2 (FADS2) gene codes for catalytic activity considered to be the rate limited step in long chain polyunsaturated fatty acid (LCPUFA) synthesis. FADS2 catalyzes 6-desaturation in at least five substrates and 8-desaturation in at least two substrates. However, the molecular mechanisms that regulate FADS2-mediated desaturation remain ill-defined. We report here characterization of an alternative transcript (AT1) of primate FADS2 and compare its expression to that of the classical transcript in 12 tissues of a 12 week old neonate baboon, and in human SK-N-SH neuroblastoma (NB) cells. RT-PCR analysis indicates relatively greater abundance of classical transcript than AT1 in all tissues. However, AT1 expression is highly variable, showing greater expression in liver, retina, occipital lobe, hippocampus, spleen, and ovary, than in other tissues, whereas classical transcript displayed little variability. These data suggest that FADS2 AT1 is a candidate for regulation of LCPUFA synthesis. |
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| معلومات مُعتمدة: | T32 DK007158 United States DK NIDDK NIH HHS; T32 HD052471 United States HD NICHD NIH HHS; T32 DK007158-34 United States DK NIDDK NIH HHS; GM071534 United States GM NIGMS NIH HHS; R01 GM071534 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (RNA, Messenger) EC 1.14.19.- (Fatty Acid Desaturases) |
| تواريخ الأحداث: | Date Created: 20090821 Date Completed: 20100930 Latest Revision: 20211020 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3119850 |
| DOI: | 10.1007/s11033-009-9750-9 |
| PMID: | 19693691 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-4978 |
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| DOI: | 10.1007/s11033-009-9750-9 |