التفاصيل البيبلوغرافية
| العنوان: |
[Detection of APC gene promoter methylation in hematological malignant cell lines by nested-methylation specific polymerase chain reaction]. |
| المؤلفون: |
Wu XM; Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujiam Province, China., Shen JZ, Yu AF, Fan LP, Zhou HR, Fu HY, Shen SF, Wu DS |
| المصدر: |
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2009 Aug; Vol. 17 (4), pp. 957-60. |
| نوع المنشور: |
English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
Chinese |
| بيانات الدورية: |
Publisher: Zhongguo shi yan xue za zhi she Country of Publication: China NLM ID: 101084424 Publication Model: Print Cited Medium: Print ISSN: 1009-2137 (Print) Linking ISSN: 10092137 NLM ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Beijing : Zhongguo shi yan xue za zhi she, |
| مواضيع طبية MeSH: |
DNA Methylation* , Genes, APC* , Promoter Regions, Genetic*, Polymerase Chain Reaction/*methods, HL-60 Cells ; Humans ; K562 Cells |
| مستخلص: |
This study was aimed to investigate the efficiency of nested methylation specific polymerase chain reaction (nMS-PCR) for detecting the APC gene promoter methylation and to clarify the roles of methylation in genesis and development of hematologic malignancies, as well as to screen the hematologic malignant cell lines with hypermethylation of APC gene promoter to use as an ideal cell model for exploring the relationship between gen methylation and gene expression. The genome DNA of 10 cell lines modified with bisulfide was amplified and the methylation status of APC gene promoter was detected by using nMS-PCR. The results showed that the methylation of APC gene promoter was detected in Jurkat cells, while could not be detected in CA46, U266, Molt4, K562, HL-60, CEM, AKR, U937 and Raji cell lines. In conclusion, APC gene methylation in hematological malignant cell lines can be accurately detected by nMS-PCP method, which is simple method for detecting methylation status of various hematological malignant cell lines. |
| تواريخ الأحداث: |
Date Created: 20090825 Date Completed: 20110713 Latest Revision: 20161018 |
| رمز التحديث: |
20231215 |
| PMID: |
19698237 |
| قاعدة البيانات: |
MEDLINE |
