دورية أكاديمية
A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.
| العنوان: | A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia. |
|---|---|
| المؤلفون: | Abodeely M; Department of Pathology, The Sandler Center for Basic Research in Parasitic Diseases, University of California, San Francisco, CA 94158, USA., DuBois KN, Hehl A, Stefanic S, Sajid M, DeSouza W, Attias M, Engel JC, Hsieh I, Fetter RD, McKerrow JH |
| المصدر: | Eukaryotic cell [Eukaryot Cell] 2009 Nov; Vol. 8 (11), pp. 1665-76. Date of Electronic Publication: 2009 Sep 11. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101130731 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-9786 (Electronic) Linking ISSN: 15359786 NLM ISO Abbreviation: Eukaryot Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Society for Microbiology, c2002- |
| مواضيع طبية MeSH: | Endoplasmic Reticulum/*metabolism , Endosomes/*metabolism , Giardia lamblia/*metabolism , Lysosomes/*metabolism, Endoplasmic Reticulum/genetics ; Endoplasmic Reticulum/ultrastructure ; Endosomes/genetics ; Endosomes/ultrastructure ; Giardia lamblia/genetics ; Giardia lamblia/ultrastructure ; Lysosomes/genetics ; Lysosomes/ultrastructure ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism |
| مستخلص: | The dynamic evolution of organelle compartmentalization in eukaryotes and how strictly compartmentalization is maintained are matters of ongoing debate. While the endoplasmic reticulum (ER) is classically envisioned as the site of protein cotranslational translocation, it has recently been proposed to have pluripotent functions. Using transfected reporter constructs, organelle-specific markers, and functional enzyme assays, we now show that in an early-diverging protozoan, Giardia lamblia, endocytosis and subsequent degradation of exogenous proteins occur in the ER or in an adjacent and communicating compartment. The Giardia endomembrane system is simple compared to those of typical eukaryotes. It lacks peroxisomes, a classical Golgi apparatus, and canonical lysosomes. Giardia orthologues of mammalian lysosomal proteases function within an ER-like tubulovesicular compartment, which itself can dynamically communicate with clathrin-containing vacuoles at the periphery of the cell to receive endocytosed proteins. These primitive characteristics support Giardia's proposed early branching and could serve as a model to study the compartmentalization of endocytic and lysosomal functions into organelles distinct from the ER. This system also may have functional similarity to the retrograde transport of toxins and major histocompatibility complex class I function in the ER of mammals. |
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| معلومات مُعتمدة: | P01 AI035707 United States AI NIAID NIH HHS; AI 35707 United States AI NIAID NIH HHS; NIHRR0450 United States PHS HHS |
| المشرفين على المادة: | 0 (Protozoan Proteins) |
| تواريخ الأحداث: | Date Created: 20090915 Date Completed: 20100114 Latest Revision: 20220330 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC2772394 |
| DOI: | 10.1128/EC.00123-09 |
| PMID: | 19749174 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1535-9786 |
|---|---|
| DOI: | 10.1128/EC.00123-09 |