دورية أكاديمية
Do Archaea and bacteria co-infection have a role in the pathogenesis of chronic chagasic cardiopathy?
| العنوان: | Do Archaea and bacteria co-infection have a role in the pathogenesis of chronic chagasic cardiopathy? |
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| المؤلفون: | Higuchi Mde L; Laboratório de Anatomia Patológica, Instituto do Coração, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil. anplourdes@incor.usp.br, Kawakami J, Ikegami R, Clementino MB, Kawamoto FM, Reis MM, Bocchi E |
| المصدر: | Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2009 Jul; Vol. 104 Suppl 1, pp. 199-207. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Instituto Oswaldo Cruz Country of Publication: Brazil NLM ID: 7502619 Publication Model: Print Cited Medium: Internet ISSN: 1678-8060 (Electronic) Linking ISSN: 00740276 NLM ISO Abbreviation: Mem Inst Oswaldo Cruz Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Rio de Janeiro : Instituto Oswaldo Cruz |
| مواضيع طبية MeSH: | Archaea/*isolation & purification , Chagas Cardiomyopathy/*microbiology , Chlamydophila pneumoniae/*isolation & purification , Complement Membrane Attack Complex/*analysis , Mycoplasma pneumoniae/*isolation & purification, Antigens, Bacterial/analysis ; Biopsy ; Chagas Cardiomyopathy/pathology ; Chronic Disease ; Humans ; In Situ Hybridization ; Microscopy, Electron ; Polymerase Chain Reaction |
| مستخلص: | Unlabelled: Chronic cardiopathy (CC) in Chagas disease is a fibrotic myocarditis with C5b-9 complement deposition. Mycoplasma and Chlamydia may interfere with the complement response. Proteolytic enzymes and archaeal genes that have been described in Trypanosoma cruzi may increase its virulence. Here we tested the hypothesis that different ratios of Mycoplasma, Chlamydia and archaeal organisms, which are frequent symbionts, may be associated with chagasic clinical forms. Materials and Methods: eight indeterminate form (IF) and 20 CC chagasic endomyocardial biopsies were submitted to in situ hybridization, electron and immunoelectron microscopy and PCR techniques for detection of Mycoplasma pneumoniae (MP), Chlamydia pneumoniae(CP), C5b-9 and archaeal-like bodies. Results: MP and CP-DNA were always present at lower levels in CC than in IF (p < 0.001) and were correlated with each other only in CC. Electron microscopy revealed Mycoplasma, Chlamydia and two types of archaeal-like bodies. One had electron dense lipid content (EDL) and was mainly present in IF. The other had electron lucent content (ELC) and was mainly present in CC. In this group, ELC correlated negatively with the other microbes and EDL and positively with C5b-9. The CC group was positive for Archaea and T. cruzi DNA. In conclusion, different amounts of Mycoplasma, Chlamydia and archaeal organisms may be implicated in complement activation and may have a role in Chagas disease outcome. |
| المشرفين على المادة: | 0 (Antigens, Bacterial) 0 (Complement Membrane Attack Complex) |
| تواريخ الأحداث: | Date Created: 20090916 Date Completed: 20100301 Latest Revision: 20190606 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/s0074-02762009000900026 |
| PMID: | 19753475 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-8060 |
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| DOI: | 10.1590/s0074-02762009000900026 |