دورية أكاديمية
Rhodococcus equi virulence-associated protein A is required for diversion of phagosome biogenesis but not for cytotoxicity.
| العنوان: | Rhodococcus equi virulence-associated protein A is required for diversion of phagosome biogenesis but not for cytotoxicity. |
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| المؤلفون: | von Bargen K; Cell Biology Institute, University of Bonn, Bonn, Germany., Polidori M, Becken U, Huth G, Prescott JF, Haas A |
| المصدر: | Infection and immunity [Infect Immun] 2009 Dec; Vol. 77 (12), pp. 5676-81. Date of Electronic Publication: 2009 Sep 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacterial Proteins/*physiology , Macrophages/*immunology , Macrophages/*microbiology , Phagosomes/*microbiology , Rhodococcus equi/*pathogenicity , Virulence Factors/*physiology, Animals ; Bacterial Proteins/genetics ; Cell Line ; Gene Knockout Techniques ; Hydrogen-Ion Concentration ; Lysosomes/physiology ; Mice ; Phagosomes/chemistry ; Phagosomes/physiology ; Rhodococcus equi/genetics ; Virulence Factors/genetics |
| مستخلص: | Rhodococcus equi is a gram-positive facultative intracellular pathogen that can cause severe bronchopneumonia in foals and AIDS patients. Virulence is plasmid regulated and is accompanied by phagosome maturation arrest and host cell necrosis. A replacement mutant in the gene for VapA (virulence-associated protein A), a major virulence factor of R. equi, was tested for its activities during macrophage infection. Early in infection, phagosomes containing the vapA mutant did not fuse with lysosomes and did not stain with the acidotropic fluor LysoTracker similar to those containing virulent wild-type R. equi. However, vapA mutant phagosomes had a lower average pH. Late in infection, phagosomes containing the vapA mutant were as frequently positive for LysoTracker as phagosomes containing plasmid-cured, avirulent bacteria, whereas those with virulent wild-type R. equi were still negative for the fluor. Macrophage necrosis after prolonged infection with virulent bacteria was accompanied by a loss of organelle staining with LysoTracker, suggesting that lysosome proton gradients had collapsed. The vapA mutant still killed the macrophages and yet did not affect the pH of host cell lysosomes. Hence, VapA is not required for host cell necrosis but is required for neutralization of phagosomes and lysosomes or their disruption. This is the first report of an R. equi mutant with altered phagosome biogenesis. |
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| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (VapA protein, Rhodococcus equi) 0 (Virulence Factors) |
| تواريخ الأحداث: | Date Created: 20091003 Date Completed: 20091204 Latest Revision: 20211020 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC2786453 |
| DOI: | 10.1128/IAI.00856-09 |
| PMID: | 19797071 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
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| DOI: | 10.1128/IAI.00856-09 |