دورية أكاديمية
أعرض في EDS

Dedifferentiation of human articular chondrocytes is associated with alterations in expression patterns of GDF-5 and its receptors.

التفاصيل البيبلوغرافية
العنوان: Dedifferentiation of human articular chondrocytes is associated with alterations in expression patterns of GDF-5 and its receptors.
المؤلفون: Schlegel W; Department of Traumatology, Medical University Vienna, Center for Joint and Cartilage Waehringer Guertel, Vienna, Austria. werner.schlegel@meduniwien.ac.at, Albrecht C, Eckl P, Freudenthaler H, Berger A, Vécsei V, Marlovits S
المصدر: Journal of cellular and molecular medicine [J Cell Mol Med] 2009 Sep; Vol. 13 (9B), pp. 3398-404. Date of Electronic Publication: 2009 Oct 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101083777 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1582-4934 (Electronic) Linking ISSN: 15821838 NLM ISO Abbreviation: J Cell Mol Med Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford, England : Wiley-Blackwell
Original Publication: Bucharest : "Carol Davila" University Press, 2000-
مواضيع طبية MeSH: Gene Expression Regulation*, Chondrocytes/*cytology , Growth Differentiation Factor 5/*biosynthesis, Aged ; Aged, 80 and over ; Bone Morphogenetic Protein Receptors, Type I/metabolism ; Carrier Proteins/biosynthesis ; Cartilage/metabolism ; Cell Differentiation ; Cell Proliferation ; Connexin 43/biosynthesis ; DNA Primers/chemistry ; Humans ; Microscopy, Fluorescence/methods ; Reverse Transcriptase Polymerase Chain Reaction
مستخلص: Human articular chondrocytes are expanded in monolayer culture in order to obtain sufficient cells for matrix-associated cartilage transplantation. During this proliferation process, the cells change their shape as well as their expression profile. These changes resemble those that occur during embryogenesis, when the limb anlagen form the interzone that later develops the joint cleft. We analysed the expression profile of genes that are reportedly important for these changes during embryogenesis within the dedifferentiation process of adult articular chondrocytes. We found GDF-5, BMPR-Ib and connexin 43 up-regulated, as well as a down-regulation of BMPR-Ia and noggin. Connexin 32 could not be detected in either native cartilage or in dedifferentiated cells. The newly synthesized proteins were detected by immunofluorescence. There is evidence from our results that dedifferentiated chondrocytes resemble the cells from the interzone in developing synovial joints.
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المشرفين على المادة: 0 (Carrier Proteins)
0 (Connexin 43)
0 (DNA Primers)
0 (GDF5 protein, human)
0 (Growth Differentiation Factor 5)
148294-77-3 (noggin protein)
EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type I)
تواريخ الأحداث: Date Created: 20091031 Date Completed: 20111223 Latest Revision: 20211020
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4516495
DOI: 10.1111/j.1582-4934.2009.00953.x
PMID: 19874419
قاعدة البيانات: MEDLINE
الوصف
تدمد:1582-4934
DOI:10.1111/j.1582-4934.2009.00953.x