دورية أكاديمية
Buckyballs meet viral nanoparticles: candidates for biomedicine.
| العنوان: | Buckyballs meet viral nanoparticles: candidates for biomedicine. |
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| المؤلفون: | Steinmetz NF; Department of Cell Biology, Center for Integrative Molecular Biosciences, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. nicoles@scripps.edu, Hong V, Spoerke ED, Lu P, Breitenkamp K, Finn MG, Manchester M |
| المصدر: | Journal of the American Chemical Society [J Am Chem Soc] 2009 Dec 02; Vol. 131 (47), pp. 17093-5. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 7503056 Publication Model: Print Cited Medium: Internet ISSN: 1520-5126 (Electronic) Linking ISSN: 00027863 NLM ISO Abbreviation: J Am Chem Soc Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Chemical Society Original Publication: Easton, Pa. [etc.] |
| مواضيع طبية MeSH: | Comovirus* , Nanoparticles*, Microscopy, Electron, Scanning Transmission |
| مستخلص: | Fullerenes such as C(60) show promise as functional components in several emerging technologies. For biomedical applications, C(60) has been used in gene- and drug-delivery vectors, as imaging agents, and as photosensitizers in cancer therapy. A major drawback of C(60) for bioapplications is its insolubility in water. To overcome this limitation, we covalently attached C(60) derivatives to Cowpea mosaic virus and bacteriophage Qbeta virus-like particles, which are examples of naturally occurring viral nanoparticle (VNP) structures that have been shown to be promising candidates for biomedicine. Two different labeling strategies were employed, giving rise to water-soluble, stable VNP-C(60) and VNP-PEG-C(60) conjugates. Samples were characterized using a combination of transmission electron microscopy, scanning transmission electron microscopy (STEM), gel electrophoresis, size-exclusion chromatography, dynamic light scattering, and Western blotting. "Click" chemistry bioconjugation using a poly(ethylene glycol) (PEG)-modified propargyl-O-PEG-C(60) derivative gave rise to high loadings of fullerene on the VNP surface, as indicated by the imaging of individual C(60) units using STEM. The cellular uptake of dye-labeled VNP-PEG-C(60) complexes in a human cancer cell line was found by confocal microscopy to be robust, showing that cell internalization was not inhibited by the attached C(60) units. These results open the door for the development of novel therapeutic devices with potential applications in photoactivated tumor therapy. |
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| معلومات مُعتمدة: | R01 RR021886-01 United States RR NCRR NIH HHS; 1K99EB009105 United States EB NIBIB NIH HHS; R01 CA112075 United States CA NCI NIH HHS; R01 CA112075-01 United States CA NCI NIH HHS; R01CA112075 United States CA NCI NIH HHS; K99 EB009105-01A1 United States EB NIBIB NIH HHS; R01 RR021886 United States RR NCRR NIH HHS; K99 EB009105 United States EB NIBIB NIH HHS; RR021886 United States RR NCRR NIH HHS |
| تواريخ الأحداث: | Date Created: 20091113 Date Completed: 20100121 Latest Revision: 20220309 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC2797550 |
| DOI: | 10.1021/ja902293w |
| PMID: | 19904938 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5126 |
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| DOI: | 10.1021/ja902293w |