دورية أكاديمية
[Production and characteristics of monoclonal antibodies to the diphtheria toxin].
| العنوان: | [Production and characteristics of monoclonal antibodies to the diphtheria toxin]. |
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| المؤلفون: | Valiakina TI; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. valyakina@ibch.ru, Lakhtina OE, Komaleva RL, Simonova MA, Samokhvalova LV, Shoshina NS, Kalinina NA, Rubina AIu, Filippova MA, Vertiev IuV, Grishin EV |
| المصدر: | Bioorganicheskaia khimiia [Bioorg Khim] 2009 Sep-Oct; Vol. 35 (5), pp. 618-28. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Nauka Country of Publication: Russia (Federation) NLM ID: 7804941 Publication Model: Print Cited Medium: Print ISSN: 0132-3423 (Print) Linking ISSN: 01323423 NLM ISO Abbreviation: Bioorg Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva, Nauka |
| مواضيع طبية MeSH: | Antibodies, Monoclonal*/biosynthesis , Antibodies, Monoclonal*/chemistry , Antibodies, Monoclonal*/immunology , Antibody Specificity*, Diphtheria Toxin/*analysis , Diphtheria Toxin/*immunology, Animals ; Bacterial Toxins/analysis ; Bacterial Toxins/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Epitopes/chemistry ; Epitopes/immunology ; Mice ; Mice, Inbred BALB C |
| مستخلص: | Monoclonal antibodies to the diphtheria toxin were produced without cross reactivity with the thermolabile toxin (LT) from Escherichia coli; ricin; choleraic toxin; the SeA, SeB, SeE, SeI, and SeG toxins of staphylococcus; the lethal factor of the anthrax toxin; and the protective antigen of the anthrax toxin. A pair of antibodies for the quantitative determination of the diphtheria toxin in the sandwich variation of enzyme-linked immunosorbent assay (ELISA) was chosen. The determination limit of the toxin was 0.7 ng/ml in plate and 1.6 ng/ml in microchip ELISA. The presence of a secretion from the nasopharynx lavage did not decrease the sensitivity of the toxin determination by sandwich ELISA. The immunization of mice with the diphtheria toxin and with a conjugate of the diphtheria toxin with polystyrene microspheres demonstrated that the conjugate immunization resulted in the formation of hybridoma clones which produced antibodies only to the epitopes of the A fragment of the diphtheria toxin. The immunization with the native toxin caused the production of hybridoma clones which predominantly produced antibodies to the epitopes of the B fragment. |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Bacterial Toxins) 0 (Diphtheria Toxin) 0 (Epitopes) |
| تواريخ الأحداث: | Date Created: 20091117 Date Completed: 20091215 Latest Revision: 20191027 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1134/s1068162009050045 |
| PMID: | 19915639 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0132-3423 |
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| DOI: | 10.1134/s1068162009050045 |