دورية أكاديمية
KLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium.
| العنوان: | KLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium. |
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| المؤلفون: | Boon RA; Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, The Netherlands., Leyen TA, Fontijn RD, Fledderus JO, Baggen JM, Volger OL, van Nieuw Amerongen GP, Horrevoets AJ |
| المصدر: | Blood [Blood] 2010 Mar 25; Vol. 115 (12), pp. 2533-42. Date of Electronic Publication: 2009 Dec 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7603509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-0020 (Electronic) Linking ISSN: 00064971 NLM ISO Abbreviation: Blood Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New York, Grune & Stratton [etc.] |
| مواضيع طبية MeSH: | Actin Cytoskeleton/*metabolism , Endothelial Cells/*metabolism , JNK Mitogen-Activated Protein Kinases/*metabolism , Kruppel-Like Transcription Factors/*metabolism , MAP Kinase Signaling System/*physiology, Activating Transcription Factor 2/metabolism ; Animals ; Aorta/cytology ; Cells, Cultured ; Endothelial Cells/cytology ; Femoral Artery/cytology ; Humans ; Kruppel-Like Transcription Factors/genetics ; Phosphorylation/physiology ; Rats ; Regional Blood Flow/physiology ; Saphenous Vein/cytology ; Stress, Mechanical ; Transduction, Genetic ; Umbilical Veins/cytology ; rho-Associated Kinases/metabolism |
| مستخلص: | The shear stress-induced transcription factor Krüppel-like factor 2 (KLF2) confers antiinflammatory properties to endothelial cells through the inhibition of activator protein 1, presumably by interfering with mitogen-activated protein kinase (MAPK) cascades. To gain insight into the regulation of these cascades by KLF2, we used antibody arrays in combination with time-course mRNA microarray analysis. No gross changes in MAPKs were detected; rather, phosphorylation of actin cytoskeleton-associated proteins, including focal adhesion kinase, was markedly repressed by KLF2. Furthermore, we demonstrate that KLF2-mediated inhibition of Jun NH(2)-terminal kinase (JNK) and its downstream targets ATF2/c-Jun is dependent on the cytoskeleton. Specifically, KLF2 directs the formation of typical short basal actin filaments, termed shear fibers by us, which are distinct from thrombin- or tumor necrosis factor-alpha-induced stress fibers. KLF2 is shown to be essential for shear stress-induced cell alignment, concomitant shear fiber assembly, and inhibition of JNK signaling. These findings link the specific effects of shear-induced KLF2 on endothelial morphology to the suppression of JNK MAPK signaling in vascular homeostasis via novel actin shear fibers. |
| المشرفين على المادة: | 0 (ATF2 protein, human) 0 (Activating Transcription Factor 2) 0 (KLF2 protein, human) 0 (Kruppel-Like Transcription Factors) EC 2.7.11.1 (rho-Associated Kinases) EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20091225 Date Completed: 20100413 Latest Revision: 20210206 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1182/blood-2009-06-228726 |
| PMID: | 20032497 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1528-0020 |
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| DOI: | 10.1182/blood-2009-06-228726 |