دورية أكاديمية
أعرض في EDS

A multidisciplinary study of archaeological grape seeds.

التفاصيل البيبلوغرافية
العنوان: A multidisciplinary study of archaeological grape seeds.
المؤلفون: Cappellini E; Department of Biology, BioArCh, University of York, P.O. Box 373, YO10 5YW, York, UK. ecappellini@googlemail.com, Gilbert MT, Geuna F, Fiorentino G, Hall A, Thomas-Oates J, Ashton PD, Ashford DA, Arthur P, Campos PF, Kool J, Willerslev E, Collins MJ
المصدر: Die Naturwissenschaften [Naturwissenschaften] 2010 Feb; Vol. 97 (2), pp. 205-17. Date of Electronic Publication: 2009 Dec 23.
نوع المنشور: Historical Article; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer Verlag Country of Publication: Germany NLM ID: 0400767 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1904 (Electronic) Linking ISSN: 00281042 NLM ISO Abbreviation: Naturwissenschaften Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Springer Verlag
مواضيع طبية MeSH: Seeds/*physiology , Vitis/*physiology, Agriculture/history ; Agriculture/methods ; Archaeology ; Climate ; DNA, Plant/genetics ; DNA, Plant/isolation & purification ; History, Medieval ; Mediterranean Region ; Plant Proteins/genetics ; Polymerase Chain Reaction/methods ; Sequence Homology, Nucleic Acid ; Vitis/classification ; Vitis/genetics ; Water Supply ; Wine
مستخلص: We report here the first integrated investigation of both ancient DNA and proteins in archaeobotanical samples: medieval grape (Vitis vinifera L.) seeds, preserved by anoxic waterlogging, from an early medieval (seventh-eighth century A.D.) Byzantine rural settlement in the Salento area (Lecce, Italy) and a late (fourteenth-fifteenth century A.D.) medieval site in York (England). Pyrolysis gas chromatography mass spectrometry documented good carbohydrate preservation, whilst amino acid analysis revealed approximately 90% loss of the original protein content. In the York sample, mass spectrometry-based sequencing identified several degraded ancient peptides. Nuclear microsatellite locus (VVS2, VVMD5, VVMD7, ZAG62 and ZAG79) analysis permitted a tentative comparison of the genetic profiles of both the ancient samples with the modern varieties. The ability to recover microsatellite DNA has potential to improve biomolecular analysis on ancient grape seeds from archaeological contexts. Although the investigation of five microsatellite loci cannot assign the ancient samples to any geographic region or modern cultivar, the results allow speculation that the material from York was not grown locally, whilst the remains from Supersano could represent a trace of contacts with the eastern Mediterranean.
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المشرفين على المادة: 0 (DNA, Plant)
0 (Plant Proteins)
تواريخ الأحداث: Date Created: 20091225 Date Completed: 20100423 Latest Revision: 20211020
رمز التحديث: 20240513
مُعرف محوري في PubMed: PMC2812422
DOI: 10.1007/s00114-009-0629-3
PMID: 20033124
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-1904
DOI:10.1007/s00114-009-0629-3