دورية أكاديمية
Analysis of uterine natural killer cells in mice.
| العنوان: | Analysis of uterine natural killer cells in mice. |
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| المؤلفون: | Croy BA; Department of Anatomy and Cell Biology, Queen's University, Kingston, ON, Canada., Zhang J, Tayade C, Colucci F, Yadi H, Yamada AT |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2010; Vol. 612, pp. 465-503. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Cytological Techniques/*methods , Killer Cells, Natural/*cytology , Uterus/*cytology, Animals ; Cell Culture Techniques ; Cell Extracts ; Female ; Flow Cytometry ; Freezing ; Humans ; Killer Cells, Natural/metabolism ; Killer Cells, Natural/ultrastructure ; Magnetics ; Male ; Mice ; Microscopy, Electron ; Nucleic Acid Amplification Techniques ; Pregnancy ; RNA/genetics ; RNA/isolation & purification ; Staining and Labeling ; Tissue Fixation |
| مستخلص: | The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment and implantation of hatched, blastocyst-stage embryos. Within each implantation site, uNK cells proliferate and rapidly differentiate into highly restricted regions called decidua basalis and the mesometrial lymphoid aggregate of pregnancy (MLAp). uNK cells begin to die within healthy decidua basalis by day 8 of the 19-20 day pregnancy of mice. By gestation day 12, uNK cell numbers have peaked and most uNK cells show in situ nuclear fragmentation indicative of disintegration. Morphological studies (standard histology, ultrastructure, immunohistochemistry, in situ hybridization, and RNA analyses from laser capture microdissected uNK cells) have provided most of the current understanding regarding this cell lineage. These approaches identified the special angiogenic properties of uNK cells and their regulatory relationships with normal physiological changes to the uterine (endometrial) arterial tree that accompany successful pregnancy. This chapter highlights key information needed for successful dissection of the dynamically changing decidua basalis that is enriched in uNK cells and special morphological procedures used for uNK cell study. Preparation of viable mouse uNK cell suspensions is difficult but can be achieved. This chapter includes techniques for isolation of uterine leukocyte suspensions and their enrichment for uNK cells that permit immediate downstream applications such as culture, isolation of high quality RNA, or flow cytometry. |
| معلومات مُعتمدة: | G0501150 United Kingdom MRC_ Medical Research Council; United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| المشرفين على المادة: | 0 (Cell Extracts) 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20091225 Date Completed: 20100226 Latest Revision: 20211028 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-60761-362-6_31 |
| PMID: | 20033660 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-60761-362-6_31 |