دورية أكاديمية
Modelling condensation and the initiation of chondrogenesis in chick wing bud mesenchymal cells levitated in an ultrasound trap.
| العنوان: | Modelling condensation and the initiation of chondrogenesis in chick wing bud mesenchymal cells levitated in an ultrasound trap. |
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| المؤلفون: | Edwards GO; Connective Tissue Biology Laboratories, Cardiff School of Biosciences, Park Place, Cardiff, Wales, CF10 3AT, UK., Coakley WT, Ralphs JR, Archer CW |
| المصدر: | European cells & materials [Eur Cell Mater] 2010 Jan 14; Vol. 19, pp. 1-12. Date of Electronic Publication: 2010 Jan 14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Forum Multimedia Publishing LLC Country of Publication: Switzerland NLM ID: 100973416 Publication Model: Electronic Cited Medium: Internet ISSN: 1473-2262 (Electronic) Linking ISSN: 14732262 NLM ISO Abbreviation: Eur Cell Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Charlottesville, VA : Forum Multimedia Publishing LLC Original Publication: [Glasgow] Scotland : European Cells & Materials, [2001]- |
| مواضيع طبية MeSH: | Chondrogenesis* , Models, Biological* , Ultrasonics*, Mesenchymal Stem Cells/*cytology , Wings, Animal/*cytology , Wings, Animal/*embryology, Actins/metabolism ; Animals ; Cell Aggregation ; Cell Nucleus/metabolism ; Cell Shape ; Chick Embryo ; Cryoultramicrotomy ; Cytoskeleton/metabolism ; Fibroblasts/cytology ; Protein Transport ; SOX9 Transcription Factor/metabolism |
| مستخلص: | Chick wing bud mesenchymal cell micromass culture allows the study of a variety of developmental mechanisms, ranging from cell adhesion to pattern formation. However, many cells remain in contact with an artificial substratum, which can influence cytoskeletal organisation and differentiation. An ultrasound standing wave trap facilitates the rapid formation of 2-D monolayer cell aggregates with a defined zero time-point, independent from contact with a surface. Aggregates formed rapidly (within 2 min) and intercellular membrane spreading occurred at points of contact. This was associated with an increase in peripheral F-actin within 10 min of cell-cell contact and aggregates had obtained physical integrity by 30 min. The chondrogenic transcription factor Sox9 could be detected in cells in the ultrasound trap within 3 h (ultrasound exposure alone was not responsible for this effect). This approach facilitates the study of the initial cell-cell interactions that occur during condensation formation and demonstrates that a combination of cell shape and cytoskeletal organisation is required for the initiation and maintenance of a differentiated phenotype, which is lost when these phenomena are influenced by contact with an artificial substrate. |
| معلومات مُعتمدة: | BB/C515220/1 United Kingdom Biotechnology and Biological Sciences Research Council |
| المشرفين على المادة: | 0 (Actins) 0 (SOX9 Transcription Factor) |
| تواريخ الأحداث: | Date Created: 20100116 Date Completed: 20100513 Latest Revision: 20220310 |
| رمز التحديث: | 20231215 |
| DOI: | 10.22203/ecm.v019a01 |
| PMID: | 20077400 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1473-2262 |
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| DOI: | 10.22203/ecm.v019a01 |