دورية أكاديمية
Assay of matrix metalloproteinases against matrix substrates.
| العنوان: | Assay of matrix metalloproteinases against matrix substrates. |
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| المؤلفون: | Cawston TE; Musculoskeletal Research Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK., Lakey RL, Rowan AD |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2010; Vol. 622, pp. 245-55. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Enzyme Assays/*methods , Matrix Metalloproteinases/*metabolism, Animals ; Cattle ; Enzyme Activation/drug effects ; Enzyme Precursors/metabolism ; Phenylmercuric Acetate/analogs & derivatives ; Phenylmercuric Acetate/pharmacology ; Staining and Labeling ; Substrate Specificity/drug effects ; Trypsin/pharmacology |
| مستخلص: | The assays described allow the activity of members of the matrix metalloproteinase (MMP) family that degrade collagen, gelatin and casein substrates to be measured. The protocols described include the preparation of radiolabeled substrates, methods for the separation of degraded product from undegraded substrate, and methods for the activation of MMPs. The advantages and disadvantages of these methods are discussed in relation to immunoassays that measure the amount of individual MMPs. |
| المشرفين على المادة: | 0 (Enzyme Precursors) 6283-24-5 (4-aminophenylmercuriacetate) EC 3.4.21.4 (Trypsin) EC 3.4.24.- (Matrix Metalloproteinases) OSX88361UX (Phenylmercuric Acetate) |
| تواريخ الأحداث: | Date Created: 20100206 Date Completed: 20100401 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-60327-299-5_15 |
| PMID: | 20135287 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-60327-299-5_15 |