دورية أكاديمية
The effect of initial tonicity on freeze/thaw injury to human red cells suspended in solutions of sodium chloride.
| العنوان: | The effect of initial tonicity on freeze/thaw injury to human red cells suspended in solutions of sodium chloride. |
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| المؤلفون: | Pegg DE; MRC Medical Cryobiology Group, University Department of Surgery, Cambridge, United Kingdom., Diaper MP |
| المصدر: | Cryobiology [Cryobiology] 1991 Feb; Vol. 28 (1), pp. 18-35. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print Cited Medium: Print ISSN: 0011-2240 (Print) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: San Diego, CA : Academic Press |
| مواضيع طبية MeSH: | Blood Preservation* , Cryopreservation* , Erythrocytes*, Hemolysis ; Humans ; In Vitro Techniques ; Osmolar Concentration ; Sodium Chloride ; Solutions |
| مستخلص: | Human red blood cells, suspended in solutions of sodium chloride, have been frozen to temperatures between -2 and -14 degrees C and thawed, and the extent of hemolysis was measured. In parallel experiments, red cells were exposed to similar cycles of change in the composition of the suspending solution, but by dialysis at 21 degrees C. The tonicity of the saline in which the cells were initially suspended was varied between 0.6x isotonic and 4x isotonic; some samples from each experimental treatment were returned to isotonic saline before hemolysis was measured. It was found that the tonicity of the saline used to suspend the cells for the main body of the experiment affected the amount of hemolysis measured: raising the tonicity from 0.6x to 1x to 2x reduced hemolysis, both in the freezing and in the dialysis experiments, whereas raising the tonicity further to 4x reversed that trend. There was little difference between the freeze/thaw and the dialysis treatments for the cells suspended in 1x or 2x saline, whether or not the cells were returned to isotonic conditions. However, the cells suspended in 0.6x saline showed greater damage from freezing and thawing than from the comparable change in the composition of the solution, whether or not they were returned to isotonic conditions. Cells that were suspended in 4x saline and exposed to changes in salt concentration by dialysis showed less hemolysis when they were assayed in the 4x solution than cells that had received the comparable freezing/thaw treatment, but when the experiment included a return to isotonicity, the two treatments gave similar results. Returning the cells to isotonic saline had a negligible affect on the cells in 0.6x and 1x saline, but caused considerable hemolysis in the 2x and 4x samples, more so after dialysis than after freezing and thawing. We conclude that cells suspended in 0.6x and 4x saline behave differently from cells suspended in 1x and 2x saline and hence that cells suspended in a range of solutions of differing initial tonicity should not be treated as a homogeneous population. We argue that an effect of the unfrozen fraction of water (U) cannot be distinguished, within the framework of these freeze/thaw experiments alone, from an effect of initial tonicity, and that the biphasic nature of the correlation between haemolysis and U makes a causal connection improbable.(ABSTRACT TRUNCATED AT 400 WORDS) |
| المشرفين على المادة: | 0 (Solutions) 451W47IQ8X (Sodium Chloride) |
| تواريخ الأحداث: | Date Created: 19910201 Date Completed: 19910520 Latest Revision: 20190912 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0011-2240(91)90004-8 |
| PMID: | 2015759 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0011-2240 |
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| DOI: | 10.1016/0011-2240(91)90004-8 |