دورية أكاديمية
Sequence analysis and characterization of a transferable hybrid plasmid encoding multidrug resistance and enabling zoonotic potential for extraintestinal Escherichia coli.
| العنوان: | Sequence analysis and characterization of a transferable hybrid plasmid encoding multidrug resistance and enabling zoonotic potential for extraintestinal Escherichia coli. |
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| المؤلفون: | Johnson TJ; Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 55108, USA., Jordan D, Kariyawasam S, Stell AL, Bell NP, Wannemuehler YM, Alarcón CF, Li G, Tivendale KA, Logue CM, Nolan LK |
| المصدر: | Infection and immunity [Infect Immun] 2010 May; Vol. 78 (5), pp. 1931-42. Date of Electronic Publication: 2010 Feb 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Drug Resistance, Multiple, Bacterial* , Plasmids*, DNA, Bacterial/*genetics , Escherichia coli/*pathogenicity , Escherichia coli Infections/*microbiology , Virulence Factors/*genetics , Zoonoses/*microbiology, Animals ; Cells, Cultured ; Chickens ; DNA, Bacterial/chemistry ; Disease Models, Animal ; Epithelial Cells/microbiology ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Female ; Genomic Islands ; Humans ; Infant, Newborn ; Male ; Molecular Sequence Data ; Rats ; Sequence Analysis, DNA ; Virulence |
| مستخلص: | ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. These plasmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avian colibacillosis, than in commensal E. coli. Here we describe pAPEC-O103-ColBM, a hybrid RepFIIA/FIB plasmid harboring components of the ColV pathogenicity island and a multidrug resistance (MDR)-encoding island. This plasmid is mobilizable and confers the ability to cause septicemia in chickens, the ability to cause bacteremia resulting in meningitis in the rat model of human disease, and the ability to resist the killing effects of multiple antimicrobial agents and human serum. The results of a sequence analysis of this and other ColV plasmids supported previous findings which indicated that these plasmid types arose from a RepFIIA/FIB plasmid backbone on multiple occasions. Comparisons of pAPEC-O103-ColBM with other sequenced ColV and ColBM plasmids indicated that there is a core repertoire of virulence genes that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningitis in a rat model. Examination of a neonatal meningitis E. coli (NMEC) population revealed that approximately 58% of the isolates examined harbored ColV-type plasmids and that 26% of these plasmids had genetic contents similar to that of pAPEC-O103-ColBM. The linkage of the ability to confer MDR and the ability contribute to multiple forms of human and animal disease on a single plasmid presents further challenges for preventing and treating ExPEC infections. |
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| سلسلة جزيئية: | GENBANK CP001232 |
| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (Escherichia coli Proteins) 0 (Virulence Factors) |
| تواريخ الأحداث: | Date Created: 20100218 Date Completed: 20100503 Latest Revision: 20211020 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC2863545 |
| DOI: | 10.1128/IAI.01174-09 |
| PMID: | 20160015 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
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| DOI: | 10.1128/IAI.01174-09 |