دورية أكاديمية
Rapid, high sensitivity, point-of-care test for cardiac troponin based on optomagnetic biosensor.
| العنوان: | Rapid, high sensitivity, point-of-care test for cardiac troponin based on optomagnetic biosensor. |
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| المؤلفون: | Dittmer WU; Philips Corporate Technologies, Eindhoven, The Netherlands. wendy.dittmer@philips.com, Evers TH, Hardeman WM, Huijnen W, Kamps R, de Kievit P, Neijzen JH, Nieuwenhuis JH, Sijbers MJ, Dekkers DW, Hefti MH, Martens MF |
| المصدر: | Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2010 Jun 03; Vol. 411 (11-12), pp. 868-73. Date of Electronic Publication: 2010 Mar 06. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1302422 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3492 (Electronic) Linking ISSN: 00098981 NLM ISO Abbreviation: Clin Chim Acta Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Biosensing Techniques/*standards , Point-of-Care Systems/*standards , Troponin I/*blood, Biosensing Techniques/methods ; Humans ; Magnetics ; Sensitivity and Specificity ; Time Factors ; Troponin I/analysis |
| مستخلص: | Background: We present a prototype handheld device based on a newly developed optomagnetic technology for the sensitive detection of cardiac troponin I (cTnI) in a finger-prick blood sample with a turnaround time of 5 min. Methods: The test was completed in a compact plastic disposable with on-board dry reagents and superparamagnetic nanoparticles. In our one-step assay, all reaction processes were precisely controlled using electromagnets positioned above and below the disposable. Nanoparticle labels (500 nm) bound to the sensor surface via a sandwich immunoassay were detected using the optical technique of frustrated total internal reflection. Results: A calibration function measured in plasma demonstrates a limit of detection (mean of blank plus 3-fold the standard deviation) of 0.03 ng/mL cTnI. A linear regression analysis of the region 0.03-6.5 ng/mL yields a slope of 37+/-4, and a linear correlation coefficient of R2=0.98. The measuring range could be extended substantially to 100 ng/mL by simultaneously imaging a second spot with a lower antibody concentration. Conclusions: The combination of magnetic particles and their fine actuation with electromagnets permits the rapid and sensitive detection of cTnI. Because of the potential high analytical performance and ease-of-use of the test, it is well suited for demanding point-of-care diagnostic applications. (Copyright 2010 Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (Troponin I) |
| تواريخ الأحداث: | Date Created: 20100310 Date Completed: 20101129 Latest Revision: 20100419 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.cca.2010.03.001 |
| PMID: | 20211616 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3492 |
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| DOI: | 10.1016/j.cca.2010.03.001 |