دورية أكاديمية
A consensus real-time RT-PCR for detection of all genotypic variants of yellow head virus of penaeid shrimp.
| العنوان: | A consensus real-time RT-PCR for detection of all genotypic variants of yellow head virus of penaeid shrimp. |
|---|---|
| المؤلفون: | Wijegoonawardane PK; CSIRO, Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Queensland 4067, Australia., Cowley JA, Walker PJ |
| المصدر: | Journal of virological methods [J Virol Methods] 2010 Jul; Vol. 167 (1), pp. 5-9. Date of Electronic Publication: 2010 Feb 26. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Penaeidae/*virology , Reverse Transcriptase Polymerase Chain Reaction/*methods , Roniviridae/*isolation & purification , Virology/*methods, Animals ; Consensus Sequence ; DNA Primers/genetics ; Roniviridae/genetics ; Sensitivity and Specificity |
| مستخلص: | A real-time quantitative (q)RT-PCR employing consensus degenerate PCR primers was developed to detect all six genotypes known currently to comprise the yellow head virus (YHV) complex and found commonly in Penaeus monodon shrimp. The test primers possess only limited (eight-fold) degeneracy and target ORF1b gene sequences identified to be highly conserved amongst 57 strains of the six genotypes detected in P. monodon sourced from various regions of the Indo-Pacific. The qRT-PCR amplifies a 147bp sequence and analysis of dilutions of synthetic genotype 2 RNA showed it to be 99.8% efficient and capable of detecting as few as 2.5 RNA copies reliably. As the test detects all six YH-complex genotypes, is extremely sensitive, capable of quantifying infection loads, and amenable to high-throughput application, it should prove useful for managing infections in P. monodon broodstock and seedstock used for aquaculture. (Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (DNA Primers) |
| تواريخ الأحداث: | Date Created: 20100312 Date Completed: 20100813 Latest Revision: 20191210 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.jviromet.2010.02.024 |
| PMID: | 20219544 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0984 |
|---|---|
| DOI: | 10.1016/j.jviromet.2010.02.024 |