دورية أكاديمية
Claudin association with CD81 defines hepatitis C virus entry.
| العنوان: | Claudin association with CD81 defines hepatitis C virus entry. |
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| المؤلفون: | Harris HJ; Institute for Biomedical Research, University of Birmingham, Birmingham B15 2TT, United Kingdom., Davis C, Mullins JG, Hu K, Goodall M, Farquhar MJ, Mee CJ, McCaffrey K, Young S, Drummer H, Balfe P, McKeating JA |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2010 Jul 02; Vol. 285 (27), pp. 21092-102. Date of Electronic Publication: 2010 Apr 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Antigens, CD/*physiology , Claudins/*genetics , Claudins/*physiology , Hepacivirus/*physiology , Hepatitis/*physiopathology, Antigens, CD/metabolism ; Cell Line ; Cholesterol/metabolism ; Claudin-1 ; DNA Primers ; Fluorescence Resonance Energy Transfer/methods ; Genes, Reporter ; HIV/enzymology ; HIV/genetics ; Hep G2 Cells/physiology ; Humans ; Luciferases/genetics ; Membrane Proteins/metabolism ; Microscopy, Confocal ; Proviruses/enzymology ; Proviruses/genetics ; Surface Plasmon Resonance ; Tetraspanin 28 ; Transfection |
| مستخلص: | Viruses initiate infection by attaching to molecules or receptors at the cell surface. Hepatitis C virus (HCV) enters cells via a multistep process involving tetraspanin CD81, scavenger receptor class B member I, and the tight junction proteins Claudin-1 and Occludin. CD81 and scavenger receptor class B member I interact with HCV-encoded glycoproteins, suggesting an initial role in mediating virus attachment. In contrast, there are minimal data supporting Claudin-1 association with HCV particles, raising questions as to its role in the virus internalization process. In the present study we demonstrate a relationship between receptor active Claudins and their association and organization with CD81 at the plasma membrane by fluorescence resonance energy transfer and stoichiometric imaging methodologies. Mutation of residues 32 and 48 in the Claudin-1 first extracellular loop ablates CD81 association and HCV receptor activity. Furthermore, mutation of the same residues in the receptor-inactive Claudin-7 molecule enabled CD81 complex formation and virus entry, demonstrating an essential role for Claudin-CD81 complexes in HCV infection. Importantly, Claudin-1 associated with CD81 at the basolateral membrane of polarized HepG2 cells, whereas tight junction-associated pools of Claudin-1 demonstrated a minimal association with CD81. In summary, we demonstrate an essential role for Claudin-CD81 complexes in HCV infection and their localization at the basolateral surface of polarized hepatoma cells, consistent with virus entry into the liver via the sinusoidal blood and association with basal expressed forms of the receptors. |
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| معلومات مُعتمدة: | G0400802 United Kingdom MRC_ Medical Research Council; G0801976 United Kingdom MRC_ Medical Research Council |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (CD81 protein, human) 0 (CLDN1 protein, human) 0 (Claudin-1) 0 (Claudins) 0 (DNA Primers) 0 (Membrane Proteins) 0 (Tetraspanin 28) 97C5T2UQ7J (Cholesterol) EC 1.13.12.- (Luciferases) |
| تواريخ الأحداث: | Date Created: 20100409 Date Completed: 20100810 Latest Revision: 20211028 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC2898367 |
| DOI: | 10.1074/jbc.M110.104836 |
| PMID: | 20375010 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1083-351X |
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| DOI: | 10.1074/jbc.M110.104836 |