دورية أكاديمية
New insights into the biological activity and secretion properties of a polypeptide derived from tilapia somatotropin.
| العنوان: | New insights into the biological activity and secretion properties of a polypeptide derived from tilapia somatotropin. |
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| المؤلفون: | Acosta J; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Carpio Y, Morales R, Aguila JC, Acanda Y, Herrera F, Estrada MP |
| المصدر: | Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2010 Aug; Vol. 156 (4), pp. 264-72. Date of Electronic Publication: 2010 Apr 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Country of Publication: England NLM ID: 9516061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1107 (Electronic) Linking ISSN: 10964959 NLM ISO Abbreviation: Comp Biochem Physiol B Biochem Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Original Publication: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994- |
| مواضيع طبية MeSH: | Tilapia*, Growth Hormone/*metabolism , Growth Hormone/*pharmacology , Peptides/*metabolism , Peptides/*pharmacology, Animals ; Cell Line ; Cell Proliferation/drug effects ; Escherichia coli/metabolism ; Goldfish/growth & development ; Growth Hormone/chemistry ; Humans ; Inclusion Bodies/metabolism ; Larva/growth & development ; Leukocytes/drug effects ; Leukocytes/metabolism ; Liver/metabolism ; Peptides/isolation & purification ; Superoxides/metabolism |
| مستخلص: | In a previous study, we unexpectedly found that tilapia growth hormone (tiGH) secreted to the culture media by transformed cells of the yeast Pichia pastoris lacks 46 amino acids from the C-terminal end. In the present study, we cloned the exact fragment that code for this truncated variant and demonstrated its growth promoting activity in goldfish when it's administered by immersion bath. Furthermore, a better characterization of this polypeptide was performed. Administration of the polypeptide derived from tiGH increased superoxide anion production and has a mitogenic effect on peripheral blood leukocytes. This molecule binds to liver membranes proteins in vitro in a saturable manner. Beside, we cloned and expressed tiGH and its truncated variant in mammalian cells using the signal peptide of this hormone and we observed that the secretion was drastically reduced in the truncated tiGH as compared to the intact molecule. Truncated tilapia growth hormone lacking the helix 4 and two disulfide loops is still a bioactive hormone, suggesting that the disulfide bonds and the helix 4 are not essential for the biological activities examined in this work. However, the growth hormone C-terminal portion seems to be essential for this hormone to be secreted by cultured cells in vitro. (2010 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Peptides) 11062-77-4 (Superoxides) 9002-72-6 (Growth Hormone) |
| تواريخ الأحداث: | Date Created: 20100413 Date Completed: 20100917 Latest Revision: 20100805 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.cbpb.2010.04.001 |
| PMID: | 20382254 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1107 |
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| DOI: | 10.1016/j.cbpb.2010.04.001 |