دورية أكاديمية
Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles.
| العنوان: | Quantitative gene monitoring of microbial tetracycline resistance using magnetic luminescent nanoparticles. |
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| المؤلفون: | Son A; Department of Civil Engineering, Auburn University, Auburn, AL 36849, USA. ason@auburn.edu, Kennedy IM, Scow KM, Hristova KR |
| المصدر: | Journal of environmental monitoring : JEM [J Environ Monit] 2010 Jun; Vol. 12 (6), pp. 1362-7. Date of Electronic Publication: 2010 Apr 28. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 100968688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-0333 (Electronic) Linking ISSN: 14640325 NLM ISO Abbreviation: J Environ Monit Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : Royal Society of Chemistry, c1999- |
| مواضيع طبية MeSH: | Environmental Monitoring*, Anti-Bacterial Agents/*pharmacology , Luminescent Agents/*chemistry , Nanoparticles/*chemistry , Nucleic Acid Hybridization/*methods , Tetracycline/*pharmacology, Drug Resistance, Microbial/genetics ; Gene Expression ; Magnetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sewage/microbiology ; Waste Disposal, Fluid |
| مستخلص: | A magnetic/luminescent nanoparticles (MLNPs) based DNA hybridization method was developed for quantitative monitoring of antibiotic resistance genes and gene-expression in environmental samples. Manipulation of magnetic field enabled the separation of the MLNPs-DNA hybrids from the solution and the fluorescence of MLNPs normalized the quantity of target DNA. In our newly developed MLNPs-DNA assay, linear standard curves (R(2) = 0.99) of target gene was determined with the detection limit of 620 gene copies. The potential risk of increased bacterial antibiotic resistance was assessed by quantitative monitoring of tetracycline resistance (i.e., tetQ gene) in wastewater microcosms. The gene abundance and its expression showed a significant increase of tetQ gene copies with the addition of tetracycline, triclosan (TCS), or triclocarban (TCC). A real-time PCR assay was employed to verify the quantification capability of the MLNPs-DNA assay and accordingly both assays have shown strong correlation (R(2) = 0.93). This non-PCR based MLNPs-DNA assay has demonstrated its potential for gene quantification via a rapid, simple, and high throughput platform and its novel use of internal calibration standards. |
| References: | J Antimicrob Chemother. 1999 Jan;43(1):5-14. (PMID: 10381095) Am J Infect Control. 2001 Oct;29(5):281-3. (PMID: 11584251) Antimicrob Agents Chemother. 1996 Nov;40(11):2562-6. (PMID: 8913465) Anal Biochem. 2007 Nov 15;370(2):186-94. (PMID: 17869209) Anal Bioanal Chem. 2008 Apr;390(7):1829-35. (PMID: 18256809) Appl Environ Microbiol. 2002 Apr;68(4):1786-93. (PMID: 11916697) J Mater Chem. 2009 Jan 1;19(27):4695-4700. (PMID: 20357905) Environ Sci Technol. 2002 Jun 1;36(11):2322-9. (PMID: 12075785) Environ Sci Technol. 2006 Jun 1;40(11):3634-9. (PMID: 16786704) Appl Environ Microbiol. 2005 Nov;71(11):6926-33. (PMID: 16269727) Appl Environ Microbiol. 2001 Apr;67(4):1494-502. (PMID: 11282596) Environ Sci Technol. 2005 Aug 1;39(15):5816-23. (PMID: 16124320) Anal Chem. 2003 Sep 15;75(18):4766-72. (PMID: 14674452) Nature. 1998 Aug 6;394(6693):531-2. (PMID: 9707111) Appl Environ Microbiol. 2001 Jan;67(1):22-32. (PMID: 11133424) Nat Mater. 2005 Nov;4(11):826-31. (PMID: 16379073) Nanotechnology. 2007 Jul;18(5):55102. (PMID: 18974844) Nat Biotechnol. 2004 Jul;22(7):883-7. (PMID: 15170215) Environ Sci Technol. 2002 Dec 1;36(23):4998-5004. (PMID: 12523412) Appl Environ Microbiol. 2000 Nov;66(11):4605-14. (PMID: 11055900) Med Res Rev. 2004 Sep;24(5):621-38. (PMID: 15224383) J Am Chem Soc. 2004 May 19;126(19):5932-3. (PMID: 15137735) Environ Sci Technol. 2002 Mar 15;36(6):1202-11. (PMID: 11944670) Environ Sci Technol. 2005 Mar 15;39(6):1420-6. (PMID: 15819193) FEMS Microbiol Lett. 2001 Aug 7;202(1):1-7. (PMID: 11506900) FEMS Microbiol Lett. 1998 Sep 15;166(2):305-9. (PMID: 9770288) Nat Biotechnol. 2001 Jul;19(7):631-5. (PMID: 11433273) Environ Sci Technol. 2004 Aug 1;38(15):4097-105. (PMID: 15352447) Nanotechnology. 2003 Dec;14(12):R63-73. (PMID: 21444976) Nano Lett. 2006 May;6(5):1059-64. (PMID: 16683851) Anal Chem. 2004 Sep 15;76(18):5302-12. (PMID: 15362886) |
| معلومات مُعتمدة: | P42 ES004699 United States ES NIEHS NIH HHS; P42 ES004699-24 United States ES NIEHS NIH HHS; 5 P42 ES004699 United States ES NIEHS NIH HHS |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Luminescent Agents) 0 (Sewage) F8VB5M810T (Tetracycline) |
| تواريخ الأحداث: | Date Created: 20100429 Date Completed: 20100914 Latest Revision: 20211020 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3267904 |
| DOI: | 10.1039/c001974g |
| PMID: | 20424797 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1464-0333 |
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| DOI: | 10.1039/c001974g |