دورية أكاديمية
High-resolution analysis of genomic alteration on chromosome arm 8p in urothelial carcinoma.
| العنوان: | High-resolution analysis of genomic alteration on chromosome arm 8p in urothelial carcinoma. |
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| المؤلفون: | Williams SV; Leeds Institute of Molecular Medicine, St James's University Hospital, Leeds, UK., Platt FM, Hurst CD, Aveyard JS, Taylor CF, Pole JC, Garcia MJ, Knowles MA |
| المصدر: | Genes, chromosomes & cancer [Genes Chromosomes Cancer] 2010 Jul; Vol. 49 (7), pp. 642-59. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 9007329 Publication Model: Print Cited Medium: Internet ISSN: 1098-2264 (Electronic) Linking ISSN: 10452257 NLM ISO Abbreviation: Genes Chromosomes Cancer Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Wiley-Liss Original Publication: New York : A.R. Liss, c1989- |
| مواضيع طبية MeSH: | Chromosome Aberrations* , Chromosomes, Human, Pair 8*, Carcinoma, Transitional Cell/*genetics , Urinary Bladder Neoplasms/*genetics, Cell Line, Tumor ; Chromosome Breakpoints ; Chromosome Deletion ; DNA Copy Number Variations ; Gene Amplification ; Humans ; Loss of Heterozygosity |
| مستخلص: | Loss of chromosome arm 8p, sometimes in combination with amplification of proximal 8p, is found in urothelial carcinoma (UC) and other epithelial cancers and is associated with more advanced tumor stage. We carried out array comparative genomic hybridization on 174 UC and 33 UC cell lines to examine breakpoints and copy number. This was followed by a detailed analysis of the cell lines using fluorescence in situ hybridization (FISH) and, in some cases, M-FISH, to refine breakpoints and determine translocation partners, heterozygosity analysis, and analysis of expression of selected genes. We showed an overall pattern of 8p loss with reduced heterozygosity and reduced gene expression. Amplification was seen in some samples and shown in the cell line JMSU1 to correlate with overexpression of ZNF703, ERLIN2, PROSC, GPR124, and BRF2. Apart from the centromere, no single breakpoint was overrepresented, and we postulate that frequent complex changes without consistent breakpoints reflect the need for alterations of combinations of genes. The region around 2 Mb, which was homozygously deleted in one cell line and includes the gene ARHGEF10 and the micro-RNA hsa-mir-596, is one candidate tumor suppressor gene region. ((c) 2010 Wiley-Liss, Inc.) |
| معلومات مُعتمدة: | C6228/A5433 United Kingdom Cancer Research UK |
| تواريخ الأحداث: | Date Created: 20100513 Date Completed: 20100909 Latest Revision: 20100512 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/gcc.20775 |
| PMID: | 20461757 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1098-2264 |
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| DOI: | 10.1002/gcc.20775 |