دورية أكاديمية
[Killing effect of cytotoxicty T lymphocyte primed by two different methods preparation of dendritic cells pulsed with antigen on Tca8113 cells in vitro].
| العنوان: | [Killing effect of cytotoxicty T lymphocyte primed by two different methods preparation of dendritic cells pulsed with antigen on Tca8113 cells in vitro]. |
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| المؤلفون: | Zhi KQ; Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital, Xi'an Jiaotong University, Xi'an 710004, China., Xu Y, Ren WH, Gao L, Zhao L, Yang Y, Zhang YC |
| المصدر: | Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology [Hua Xi Kou Qiang Yi Xue Za Zhi] 2010 Apr; Vol. 28 (2), pp. 195-8. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Sichuan yi xue yuan Country of Publication: China NLM ID: 9422648 Publication Model: Print Cited Medium: Print ISSN: 1000-1182 (Print) Linking ISSN: 10001182 NLM ISO Abbreviation: Hua Xi Kou Qiang Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Chengdu : Sichuan yi xue yuan Original Publication: Chengdu : Sichuan yi xue yuan, 1983- |
| مواضيع طبية MeSH: | Dendritic Cells* , T-Lymphocytes, Cytotoxic*, Humans ; In Vitro Techniques ; Tongue Neoplasms |
| مستخلص: | Objective: To study the feasibility of dendritic cells (DCs) vaccine on the therapy of tongue carcinoma and find the better way of antigen load. Methods: The antigen peptides of Tca8113 cells were obtained by acid eluted technique and repetitive freeze thaw method. Separating T cell and inducing dendritic cells were obtained from human peripheral blood monocyte. Divided into three groups: Weak acid elution method antigen group, anti-freeze-thaw method antigen group, and the control group (without tumor antigen). T cells and UCs were mixed to culture by different effector-target ratio. Using MTT assay measured the quantities of absorbance and calculated stimulation index. Dendrtic cells pulsed with antigen were mixed with T cells by different effector-target ratio. MIT assay was used to measure the quantities of absorbance and calculate killing rate. Results: DCs vaccine was constructed successfully. DCs vaccine can induce T lymphocytes to kill Tca8113 cells and display the dose-effect relationship. There was significant difference among the three groups. The acid eluted and repetitive freeze thaw groups were better than the control group. The acid eluted group was better than repetitive freeze thaw group. Conclusion: DCs vaccine can induce T lymphocytes to kill Tca8113 cells. The antigen peptides obtained by acid eluted technique is better than repetitive freeze thaw method in immunotherapy of tongue cancer. |
| تواريخ الأحداث: | Date Created: 20100520 Date Completed: 20150607 Latest Revision: 20181201 |
| رمز التحديث: | 20231215 |
| PMID: | 20480667 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-1182 |
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