دورية أكاديمية
[Standardization and use of an immunoenzymatic method using infected cells as antigens in routine diagnosing of dengue].
| العنوان: | [Standardization and use of an immunoenzymatic method using infected cells as antigens in routine diagnosing of dengue]. |
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| عنوان ترانسليتريتد: | Padronização e uso de um método imunoenzimático que utiliza células infectadas como antígeno no diagnóstico rotineiro do dengue. |
| المؤلفون: | Barreira LA; Centro de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP., Machado AM, Aquino VH, Badra SJ, Figueiredo LT |
| المصدر: | Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2010 May-Jun; Vol. 43 (3), pp. 268-71. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Portuguese |
| بيانات الدورية: | Publisher: Sociedade Brasileira de Medicina Tropical Country of Publication: Brazil NLM ID: 7507456 Publication Model: Print Cited Medium: Internet ISSN: 1678-9849 (Electronic) Linking ISSN: 00378682 NLM ISO Abbreviation: Rev Soc Bras Med Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1997-> : Uberaba, MG, Brazil : Sociedade Brasileira de Medicina Tropical Original Publication: Rio De Janeiro : Sociedade Brasileira De Medicina Tropical |
| مواضيع طبية MeSH: | Antigens, Viral*/immunology, Antibodies, Viral/*blood , Dengue/*diagnosis , Dengue Virus/*immunology , Immunoenzyme Techniques/*standards, Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antibodies, Viral/immunology ; Cell Line ; Child ; Child, Preschool ; Dengue/immunology ; Female ; Humans ; Immunoenzyme Techniques/methods ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Immunoglobulin M/blood ; Immunoglobulin M/immunology ; Male ; Middle Aged ; Sensitivity and Specificity ; Young Adult |
| مستخلص: | Introduction: This paper show the standardization and use of the immunoenzymatic method using infected cells as antigens (EIA-ICC) for routine serological diagnosing of dengue. Methods: In optimizing the test, a dose of 1,000 TCID50 of dengue type 3 virus (DENV-3) was used, and 100,000 C636 cells infected with 1,000 TCID50 (DENV-3) were used. Results: The results obtained with EIA-ICC were compared with the HUMAN commercial dengue kit. The results were highly concordant. The EIA-ICC showed moderate sensitivity and high specificity. The test was used for serologically diagnosing 1,797 blood samples from suspected dengue cases during the 2006 epidemic in Ribeirao Preto. From the serological evaluation, 228 samples were positive for IgM against DENV-3; 235 samples were positive for IgG against DENV-3; and 35 samples were positive for both IgG and IgM. Conclusions: EIA-ICC was shown to be reliable and simple, and suitable for serologically diagnosing dengue. |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Antigens, Viral) 0 (Immunoglobulin G) 0 (Immunoglobulin M) |
| تواريخ الأحداث: | Date Created: 20100622 Date Completed: 20110105 Latest Revision: 20190907 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/s0037-86822010000300011 |
| PMID: | 20563494 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-9849 |
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| DOI: | 10.1590/s0037-86822010000300011 |