دورية أكاديمية
Exchange of chromosomal markers by natural transformation between the soil isolate, Pseudomonas stutzeri JM300, and the marine isolate, Pseudomonas stutzeri strain ZoBell.
| العنوان: | Exchange of chromosomal markers by natural transformation between the soil isolate, Pseudomonas stutzeri JM300, and the marine isolate, Pseudomonas stutzeri strain ZoBell. |
|---|---|
| المؤلفون: | Stewart GJ; Department of Biology, University of South Florida, Tampa 33620., Sinigalliano CD |
| المصدر: | Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 1991 Jan; Vol. 59 (1), pp. 19-25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 0372625 Publication Model: Print Cited Medium: Print ISSN: 0003-6072 (Print) Linking ISSN: 00036072 NLM ISO Abbreviation: Antonie Van Leeuwenhoek Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2004- : Berlin : Springer Original Publication: Wageningen, Netherland [etc.] Veenman [etc.] |
| مواضيع طبية MeSH: | Genetic Markers* , Transformation, Bacterial*, Pseudomonas/*genetics, Deoxyribonuclease I/metabolism ; Drug Resistance, Microbial/genetics ; Genes, Bacterial ; Nalidixic Acid/pharmacology ; Rifampin/pharmacology ; Streptomycin/pharmacology |
| مستخلص: | Both the soil isolate, Pseudomonas stutzeri JM300, and the marine isolate, Pseudomonas stutzeri strain ZoBell, have been shown previously to be naturally transformable. This study reports the detection of genetic exchange by natural transformation between these two isolates. Transformation frequency was determined by filter transformation procedures. Three independent antibiotic resistance loci were used as chromosomal markers to monitor this exchange event: resistance to rifampicin, streptomycin, and nalidixic acid. The maximum frequencies of transformation were on the order of 3.1 to 3.8 x 10(-6) transformants per recipient; frequencies over an order of magnitude greater than those for spontaneous antibiotic resistance, although they are lower than those observed for soil:soil or marine:marine strain crosses. This exchange was inhibited by DNase I. Transformation was observed between soil and marine strains, both by filter transformation using purified DNA solutions and when transforming DNA was added in the form of viable donor cells. The results from this study support the close genetic relationship between P. stutzeri JM300 and P. stutzeri strain ZoBell. These results also further validate the utility of P. stutzeri as a benchmark organism for modeling gene transfer by natural transformation in both soil and marine habitats. |
| References: | J Bacteriol. 1972 Nov;112(2):917-31. (PMID: 4563985) J Hyg (Lond). 1928 Jan;27(2):113-59. (PMID: 20474956) J Exp Med. 1944 Feb 1;79(2):137-58. (PMID: 19871359) Annu Rev Biochem. 1981;50:41-68. (PMID: 7023362) Biochim Biophys Acta. 1979 Dec 6;548(3):484-99. (PMID: 228713) Appl Environ Microbiol. 1990 Jun;56(6):1818-24. (PMID: 16348222) Appl Environ Microbiol. 1983 Apr;45(4):1247-53. (PMID: 6407395) Appl Environ Microbiol. 1987 Dec;53(12):2948-52. (PMID: 3435148) J Membr Biol. 1984;81(2):89-103. (PMID: 6387128) Annu Rev Microbiol. 1986;40:211-35. (PMID: 3535646) J Bacteriol. 1967 Sep;94(3):562-70. (PMID: 4962301) Mol Gen Genet. 1983;191(1):39-45. (PMID: 6412037) Appl Environ Microbiol. 1982 Jun;43(6):1393-9. (PMID: 16346035) J Bacteriol. 1986 Sep;167(3):1074-6. (PMID: 3091578) Antonie Van Leeuwenhoek. 1989;55(1):15-22. (PMID: 2662898) Appl Environ Microbiol. 1987 Jan;53(1):170-9. (PMID: 3827244) J Bacteriol. 1982 Mar;149(3):816-23. (PMID: 7061387) J Bacteriol. 1984 Apr;158(1):36-42. (PMID: 6425267) Appl Environ Microbiol. 1983 Aug;46(2):417-20. (PMID: 16346365) J Bacteriol. 1983 Oct;156(1):30-5. (PMID: 6194148) J Bacteriol. 1983 Jan;153(1):93-9. (PMID: 6571730) J Gen Microbiol. 1966 May;43(2):273-92. (PMID: 5962361) |
| المشرفين على المادة: | 0 (Genetic Markers) 3B91HWA56M (Nalidixic Acid) EC 3.1.21.1 (Deoxyribonuclease I) VJT6J7R4TR (Rifampin) Y45QSO73OB (Streptomycin) |
| تواريخ الأحداث: | Date Created: 19910101 Date Completed: 19910801 Latest Revision: 20190828 |
| رمز التحديث: | 20221208 |
| DOI: | 10.1007/BF00582115 |
| PMID: | 2059008 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0003-6072 |
|---|---|
| DOI: | 10.1007/BF00582115 |