دورية أكاديمية
Amino acids change liver growth factors gene expression in malnourished rats.
| العنوان: | Amino acids change liver growth factors gene expression in malnourished rats. |
|---|---|
| المؤلفون: | Passos de Jesus R; Nutrition Science Department, Nutrition School, Federal University of Bahia, Bahia, Brazil., De Nardi L, Da Rós N, Salaorni S, Nagai MA, Mitzi Brentani M, Akamine D, Waitzberg DL |
| المصدر: | Nutricion hospitalaria [Nutr Hosp] 2010 May-Jun; Vol. 25 (3), pp. 382-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Grupo Aran Country of Publication: Spain NLM ID: 9100365 Publication Model: Print Cited Medium: Internet ISSN: 1699-5198 (Electronic) Linking ISSN: 02121611 NLM ISO Abbreviation: Nutr Hosp Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : Madrid : Grupo Aran Original Publication: Madrid : Jarpyo Editores, |
| مواضيع طبية MeSH: | Gene Expression Regulation*/drug effects, Amino Acids/*pharmacology , Intercellular Signaling Peptides and Proteins/*genetics , Malnutrition/*genetics, Amino Acids/physiology ; Animals ; Intercellular Signaling Peptides and Proteins/biosynthesis ; Liver/metabolism ; Male ; Rats ; Rats, Wistar |
| مستخلص: | Background: Glutamine and proline are metabolized the liver and may collaborate on its regeneration. Parenteral nutrition (PN) containing either glutamine or proline was given to partially hepatectomized rats. The total RNA content and growth factor gene expression in hepatic remnants was measured, to determine the effects of these amino acid supplementation on the expression of growth factors during liver regeneration. Methods: Wistar rats nourished (HN) and malnourished (HM) were hepatectomized and divided in two groups: 20 receiving PN enriched with Alanyl-Glutamine (HN-Gln and HM-Gln) and 20 PN enriched with proline+alanine (HN-Pro and HM-Pro). The control groups comprised 7 nourished (CN) and 7 malnourished (CM) rats that didn't undergo surgery. Growth factor and thymidine kinase mRNA levels were measured by RT-PCR. Results: In nourished rats, total hepatic RNA levels were lower in the HN-Gln and HN-Pro groups (0.75 and 0.63 microg/mg tissue, respectively) than in control group (1.67 microg/mg tissue) (P<0.05). In malnourished rats, total hepatic RNA content was higher in the HM-Pro group than HN-Pro, HM-Gln, and CM (3.18 vs. 0.63, 0.93 and 1.10 microg/mg, respectively; P<0.05). Hepatocyte growth factor mRNA was more abundant in the HM-Gln group when compared to CM (0.31 vs. 0.23 arbitrary units) and also in HM-Pro in relation to HM-Gln, HN-Pro, and CM(0.46 vs. 0.33 and 0.23, respectively, P<0.05). Conclusions: Proline or glutamine supplementation in malnourished rats improves total RNA content in the remnant hepatic tissue. Amino acids administration increased HGF gene expression after partial hepatectomy in malnourished rats, with a greater effect of proline than glutamine. |
| المشرفين على المادة: | 0 (Amino Acids) 0 (Intercellular Signaling Peptides and Proteins) |
| تواريخ الأحداث: | Date Created: 20100702 Date Completed: 20110201 Latest Revision: 20130702 |
| رمز التحديث: | 20240628 |
| PMID: | 20593119 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1699-5198 |
|---|