دورية أكاديمية
Hyperosmotic Stimulus Down-regulates 1alpha, 25-dihydroxyvitamin D(3)-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System.
| العنوان: | Hyperosmotic Stimulus Down-regulates 1alpha, 25-dihydroxyvitamin D(3)-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System. |
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| المؤلفون: | Tian YS; Department of Oral Biology, Yonsei University, Seoul 120-752, Korea., Jeong HJ, Lee SD, Kong SH, Ohk SH, Yoo YJ, Seo JT, Shin DM, Sohn BW, Lee SI |
| المصدر: | The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology [Korean J Physiol Pharmacol] 2010 Jun; Vol. 14 (3), pp. 169-76. Date of Electronic Publication: 2010 Jun 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Physiological Society [and] Korean Society of Pharmacology Country of Publication: Korea (South) NLM ID: 9709505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2093-3827 (Electronic) Linking ISSN: 12264512 NLM ISO Abbreviation: Korean J Physiol Pharmacol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Seoul : Korean Physiological Society [and] Korean Society of Pharmacology, 1997- |
| مستخلص: | The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1alpha, 25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3))-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1alpha,25(OH)(2)D(3)-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1alpha,25(OH)(2)D(3)-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1alpha,25(OH)(2)D(3)-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor kappaB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1alpha,25(OH)(2)D(3). Knockdown of Runx2 inhibited 1alpha,25(OH)(2)D(3)-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1alpha,25(OH)(2)D(3)-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells. |
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| فهرسة مساهمة: | Keywords: Hyperosmotic stimulus; Osteoblast; RANKL; Runx2; TonEBP |
| تواريخ الأحداث: | Date Created: 20100716 Date Completed: 20110714 Latest Revision: 20211020 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC2902809 |
| DOI: | 10.4196/kjpp.2010.14.3.169 |
| PMID: | 20631890 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2093-3827 |
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| DOI: | 10.4196/kjpp.2010.14.3.169 |