دورية أكاديمية
Defined particle ligands trigger specific defense mechanisms of macrophages.
| العنوان: | Defined particle ligands trigger specific defense mechanisms of macrophages. |
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| المؤلفون: | Dykstra T; Cell Biology Institute, University of Bonn, Germany., Utermoehlen O, Haas A |
| المصدر: | Innate immunity [Innate Immun] 2011 Aug; Vol. 17 (4), pp. 388-402. Date of Electronic Publication: 2010 Aug 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Publications Country of Publication: United States NLM ID: 101469670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1753-4267 (Electronic) Linking ISSN: 17534259 NLM ISO Abbreviation: Innate Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Los Angeles : Sage Publications, c2008- |
| مواضيع طبية MeSH: | Phagocytosis*/immunology, Escherichia coli/*immunology , Macrophages/*metabolism, Animals ; Complement C1q/immunology ; Complement C1q/metabolism ; Complement C3b/immunology ; Complement C3b/metabolism ; Cytokines/metabolism ; Cytokines/pharmacology ; Cytotoxicity, Immunologic/drug effects ; Female ; Fibronectins/immunology ; Fibronectins/metabolism ; Immunity, Innate ; Immunoglobulin G/immunology ; Immunoglobulin G/metabolism ; Macrophages/immunology ; Macrophages/pathology ; Mannans/immunology ; Mannans/metabolism ; Mice ; Mice, Inbred C57BL ; Microspheres ; beta-Glucans/immunology ; beta-Glucans/metabolism |
| مستخلص: | Phagocytosis is a receptor-mediated process for sequestration and inactivation of infectious microbes. It can be triggered by microbial surface compounds or particle-attached host proteins. We monitored the effector functions of murine bone marrow-derived macrophages (BMMs) in response to polystyrene-streptavidin beads coated with the defined ligands IgG1, β-glucan, mannan, complement factors C1q or iC3b, or fibronectin (FN). Cell-autonomous effector mechanisms (uptake, phagosome maturation, cytokine responses and killing activity) were differentially triggered. All particle-ligand complexes stimulated the release of nitric oxide, but only beads coated with IgG, complement factors or FN caused production of superoxide. Beads coated with C1q, iC3b or FN strongly stimulated the secretion of pro-inflammatory TNF-α, IL-6, and IL-1β and also of anti-inflammatory IL-10. Escherichia coli coated with C1q, iC3b or FN was killed much less efficiently than with any of the other ligands, depending on the presence of IL-10 activity. This indicated an important role of IL-10 as regulator of cell-autonomous immune functions of macrophages. Our data show that defined ligands on microbial surfaces are interesting candidates to activate innate defense mechanisms selectively and specifically. |
| المشرفين على المادة: | 0 (Cytokines) 0 (Fibronectins) 0 (Immunoglobulin G) 0 (Mannans) 0 (beta-Glucans) 80295-33-6 (Complement C1q) 80295-43-8 (Complement C3b) |
| تواريخ الأحداث: | Date Created: 20100805 Date Completed: 20111121 Latest Revision: 20181201 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1177/1753425910374889 |
| PMID: | 20682584 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1753-4267 |
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| DOI: | 10.1177/1753425910374889 |