دورية أكاديمية
أعرض في EDS

Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment.

التفاصيل البيبلوغرافية
العنوان: Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment.
المؤلفون: Overmeer RM; Department of Toxicogenetics, Leiden University Medical Center, Leiden, Netherlands., Gourdin AM, Giglia-Mari A, Kool H, Houtsmuller AB, Siegal G, Fousteri MI, Mullenders LH, Vermeulen W
المصدر: Molecular and cellular biology [Mol Cell Biol] 2010 Oct; Vol. 30 (20), pp. 4828-39. Date of Electronic Publication: 2010 Aug 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 8109087 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5549 (Electronic) Linking ISSN: 02707306 NLM ISO Abbreviation: Mol Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2023- : [Philadelphia] : Taylor & Francis
Original Publication: [Washington, D.C.] : American Society for Microbiology, [c1981-
مواضيع طبية MeSH: DNA Polymerase III/*metabolism , DNA Repair/*physiology , Replication Protein C/*metabolism, Binding Sites ; Cell Line ; Cytarabine/pharmacology ; DNA Damage ; DNA Replication ; Fluorescence Recovery After Photobleaching ; Gene Knockdown Techniques ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Humans ; Hydroxyurea/pharmacology ; Kinetics ; Models, Biological ; Nucleic Acid Synthesis Inhibitors/pharmacology ; Proliferating Cell Nuclear Antigen/metabolism ; RNA, Small Interfering/genetics ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Replication Protein C/antagonists & inhibitors ; Replication Protein C/genetics ; Ultraviolet Rays
مستخلص: Nucleotide excision repair (NER) operates through coordinated assembly of repair factors into pre- and postincision complexes. The postincision step of NER includes gap-filling DNA synthesis and ligation. However, the exact composition of this NER-associated DNA synthesis complex in vivo and the dynamic interactions of the factors involved are not well understood. Using immunofluorescence, chromatin immunoprecipitation, and live-cell protein dynamic studies, we show that replication factor C (RFC) is implicated in postincision NER in mammalian cells. Small interfering RNA-mediated knockdown of RFC impairs upstream removal of UV lesions and abrogates the downstream recruitment of DNA polymerase delta. Unexpectedly, RFC appears dispensable for PCNA recruitment yet is required for the subsequent recruitment of DNA polymerases to PCNA, indicating that RFC is essential to stably load the polymerase clamp to start DNA repair synthesis at 3' termini. The kinetic studies are consistent with a model in which RFC exchanges dynamically at sites of repair. However, its persistent localization at stalled NER complexes suggests that RFC remains targeted to the repair complex even after loading of PCNA. We speculate that RFC associates with the downstream 5' phosphate after loading; such interaction would prevent possible signaling events initiated by the RFC-like Rad17 and may assist in unloading of PCNA.
References: Annu Rev Biochem. 1998;67:721-51. (PMID: 9759502)
Genes Dev. 2000 Feb 1;14(3):349-59. (PMID: 10673506)
Mol Cell. 2001 Jul;8(1):213-24. (PMID: 11511374)
J Cell Sci. 2008 Sep 1;121(Pt 17):2850-9. (PMID: 18682493)
Cell Growth Differ. 1996 Mar;7(3):319-26. (PMID: 8838862)
EMBO J. 2003 Oct 1;22(19):5163-74. (PMID: 14517254)
J Mol Med (Berl). 1999 Apr;77(4):386-92. (PMID: 10353443)
EMBO J. 2009 Apr 22;28(8):1111-20. (PMID: 19279666)
Nucleic Acids Res. 2005 Jul 21;33(13):4078-89. (PMID: 16040599)
J Cell Biol. 2000 Apr 17;149(2):271-80. (PMID: 10769021)
Genes Cells. 1996 Jan;1(1):101-13. (PMID: 9078370)
EMBO Rep. 2001 Nov;2(11):1013-7. (PMID: 11713193)
Curr Biol. 2003 Nov 11;13(22):R873-5. (PMID: 14614842)
EMBO J. 2008 Jan 9;27(1):155-67. (PMID: 18079701)
J Biol Chem. 2006 Nov 17;281(46):35531-43. (PMID: 16980295)
J Biol Chem. 2006 Feb 17;281(7):4308-17. (PMID: 16361700)
Nucleic Acids Res. 2007;35(9):2913-23. (PMID: 17439963)
Mol Cell Biol. 1989 Feb;9(2):609-19. (PMID: 2565531)
PLoS Biol. 2003 Nov;1(2):E33. (PMID: 14624239)
DNA Repair (Amst). 2005 May 2;4(5):571-82. (PMID: 15811629)
Genes Cells. 2004 Apr;9(4):279-90. (PMID: 15066120)
Mol Cell Biol. 2005 Jul;25(13):5445-55. (PMID: 15964801)
Curr Biol. 2003 Sep 16;13(18):1583-95. (PMID: 13678589)
Cell. 1995 Mar 24;80(6):859-68. (PMID: 7697716)
J Biol Chem. 1997 Sep 26;272(39):24522-9. (PMID: 9305916)
Adv Biochem Eng Biotechnol. 2005;95:177-99. (PMID: 16080269)
J Biol Chem. 2003 Nov 21;278(47):46906-10. (PMID: 12944386)
Mol Cell Biol. 2005 Nov;25(21):9350-9. (PMID: 16227586)
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1633-8. (PMID: 12578958)
DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1009-14. (PMID: 15279787)
J Biol Chem. 1998 Nov 27;273(48):31992-9. (PMID: 9822671)
Biochim Biophys Acta. 1985 Oct 3;826(1):38-48. (PMID: 2994738)
Biochemistry. 1984 Mar 27;23(7):1383-91. (PMID: 6426505)
Nature. 2001 May 17;411(6835):366-74. (PMID: 11357144)
J Cell Biol. 2004 Jul 5;166(1):27-36. (PMID: 15226310)
Neuroscience. 2007 Apr 14;145(4):1388-96. (PMID: 17276014)
Nat Rev Mol Cell Biol. 2008 Dec;9(12):958-70. (PMID: 19023283)
Mol Cell. 2006 Aug;23(4):471-82. (PMID: 16916636)
Chem Rev. 2006 Feb;106(2):253-76. (PMID: 16464005)
J Biol Chem. 1997 Jan 10;272(2):1256-62. (PMID: 8995429)
Nucleic Acids Res. 1998 Sep 1;26(17):3877-82. (PMID: 9705493)
Mol Cell. 2010 Mar 12;37(5):714-27. (PMID: 20227374)
J Cell Biol. 2010 May 3;189(3):445-63. (PMID: 20439997)
EMBO J. 1999 Nov 1;18(21):6189-99. (PMID: 10545128)
EMBO J. 1995 Jan 16;14(2):360-7. (PMID: 7835346)
Mol Cell. 2007 Jul 20;27(2):311-23. (PMID: 17643379)
Mutat Res. 1979 Feb;59(2):257-71. (PMID: 35744)
المشرفين على المادة: 0 (Nucleic Acid Synthesis Inhibitors)
0 (Proliferating Cell Nuclear Antigen)
0 (RFC1 protein, human)
0 (RNA, Small Interfering)
0 (Recombinant Fusion Proteins)
04079A1RDZ (Cytarabine)
147336-22-9 (Green Fluorescent Proteins)
EC 2.7.7.7 (DNA Polymerase III)
EC 3.6.4.- (Replication Protein C)
X6Q56QN5QC (Hydroxyurea)
تواريخ الأحداث: Date Created: 20100818 Date Completed: 20101028 Latest Revision: 20211020
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC2950542
DOI: 10.1128/MCB.00285-10
PMID: 20713449
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5549
DOI:10.1128/MCB.00285-10