دورية أكاديمية
أعرض في EDS

Inflammatory conditions affect gene expression and function of human adipose tissue-derived mesenchymal stem cells.

التفاصيل البيبلوغرافية
العنوان: Inflammatory conditions affect gene expression and function of human adipose tissue-derived mesenchymal stem cells.
المؤلفون: Crop MJ; Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands. m.crop@erasmusmc.nl, Baan CC, Korevaar SS, Ijzermans JN, Pescatori M, Stubbs AP, van Ijcken WF, Dahlke MH, Eggenhofer E, Weimar W, Hoogduijn MJ
المصدر: Clinical and experimental immunology [Clin Exp Immunol] 2010 Dec; Vol. 162 (3), pp. 474-86. Date of Electronic Publication: 2010 Sep 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0057202 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2249 (Electronic) Linking ISSN: 00099104 NLM ISO Abbreviation: Clin Exp Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2022- : Oxford : Oxford University Press
Original Publication: Oxford : Blackwell Scientific Publications
مواضيع طبية MeSH: Cyclooxygenase 2/*biosynthesis , Cytokines/*pharmacology , Indoleamine-Pyrrole 2,3,-Dioxygenase/*metabolism , Inflammation Mediators/*pharmacology , Mesenchymal Stem Cells/*drug effects, Adipose Tissue/cytology ; Cell Proliferation ; Cells, Cultured ; Coculture Techniques ; Cyclooxygenase 2/genetics ; Gene Expression Profiling ; Gene Expression Regulation/immunology ; Humans ; Immunosuppression Therapy ; Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics ; Indoleamine-Pyrrole 2,3,-Dioxygenase/immunology ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/metabolism ; Lymphocyte Activation ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/immunology ; Mesenchymal Stem Cells/metabolism
مستخلص: There is emerging interest in the application of mesenchymal stem cells (MSC) for the prevention and treatment of autoimmune diseases, graft-versus-host disease and allograft rejection. It is, however, unknown how inflammatory conditions affect phenotype and function of MSC. Adipose tissue-derived mesenchymal stem cells (ASC) were cultured with alloactivated peripheral blood mononuclear cells (PBMC) (mixed lymphocyte reaction: MLR), with proinflammatory cytokines [interferon (IFN)-γ, tumour necrosis factor (TNF)-α and interleukin (IL)-6] or under control conditions, and their full genome expression and function examined. Proinflammatory cytokines mainly increased indoleamine-2,3-dioxygenase expression, whereas ASC cultured with MLR showed increased expression of COX-2, involved in prostaglandin E(2) production. Both conditions had a stimulatory, but differential, effect on the expression of proinflammatory cytokines and chemokines, while the expression of fibrotic factors was decreased only in response to proinflammatory cytokines. Functional analysis demonstrated that inflammatory conditions affected morphology and proliferation of ASC, while their differentiation capacity and production of trophic factors was unaffected. The immunosuppressive capacity of ASC was enhanced strongly under inflammatory conditions. In conclusion, ASC showed enhanced immunosuppressive capacity under inflammatory conditions, while their differentiation capacity was preserved. Therefore, in vitro preconditioning provides ASC with improved properties for immediate clinical immune therapy.
(© 2010 Authors. Clinical and Experimental Immunology © 2010 British Society for Immunology.)
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سلسلة جزيئية: GEO GSE18662
المشرفين على المادة: 0 (Cytokines)
0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
0 (Inflammation Mediators)
EC 1.14.99.1 (Cyclooxygenase 2)
تواريخ الأحداث: Date Created: 20100918 Date Completed: 20110308 Latest Revision: 20220330
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC3026550
DOI: 10.1111/j.1365-2249.2010.04256.x
PMID: 20846162
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2249
DOI:10.1111/j.1365-2249.2010.04256.x