دورية أكاديمية
[Construction and identification of siRNA recombinant expression vector targeting parathyroid hormone 1 receptor gene and its effect on the cell cycle of INS-1 cells].
| العنوان: | [Construction and identification of siRNA recombinant expression vector targeting parathyroid hormone 1 receptor gene and its effect on the cell cycle of INS-1 cells]. |
|---|---|
| المؤلفون: | Liang HS; Department of Endocrinology, Ninth Affiliated Hospital of Guangxi Medical University, Beihai 536000, China. flowchaos@yahoo.com.cn, Xue YM, Zhong YH |
| المصدر: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2010 Sep; Vol. 30 (9), pp. 2093-6. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Nanfang yi ke da xue xue bao bian ji bu Country of Publication: China NLM ID: 101266132 Publication Model: Print Cited Medium: Print ISSN: 1673-4254 (Print) Linking ISSN: 16734254 NLM ISO Abbreviation: Nan Fang Yi Ke Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou : Nanfang yi ke da xue xue bao bian ji bu, 2005- |
| مواضيع طبية MeSH: | Cell Cycle/*drug effects , Genetic Vectors/*genetics , Glucose/*pharmacology , Insulin-Secreting Cells/*cytology , Receptor, Parathyroid Hormone, Type 1/*genetics, Humans ; Insulin-Secreting Cells/drug effects ; Insulin-Secreting Cells/metabolism ; RNA, Small Interfering/genetics ; Receptor, Parathyroid Hormone, Type 1/metabolism |
| مستخلص: | Objective: To construct the expression vector of siRNA targeting parathyroid hormone 1 receptor (PTH1R) gene and evaluate its effect on the cell cycle of INS-1 cells. Methods: The sequences of PTH1R gene was retrieved from Genbank, and 4 pairs of oligonucleotides were synthesized and inserted into pSUPERretro RNAi, which was identified by RT-PCR and sequence analysis. The vectors were then transfected into INS-1 cells, in which the expression of PTH1R was observed by Western blotting to evaluate the transfection efficiency. The cell cycle of INS-1 cells in high glucose medium was detected by flow cytometry. Results: RT-PCR and sequence analysis confirmed the correct construction of the siRNA recombinant expression vector targeting PTH1R gene. The vectors were successfully transfected into INS-1 cells, and the most effective vector was selected by Western blotting. Transfection with the siRNA for PTH1R gene silencing resulted in the inhibition of INS-1 form entering the S phase. Conclusion: The successful construction of the recombinant PTH1R-siRNA vectors establishes a basis for further study of protective role of the PTH1R gene in INS-1 cells in high glucose medium. |
| المشرفين على المادة: | 0 (RNA, Small Interfering) 0 (Receptor, Parathyroid Hormone, Type 1) IY9XDZ35W2 (Glucose) |
| تواريخ الأحداث: | Date Created: 20100922 Date Completed: 20111128 Latest Revision: 20140919 |
| رمز التحديث: | 20221213 |
| PMID: | 20855259 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1673-4254 |
|---|