دورية أكاديمية
Two different spectrofluorimetric methods for simultaneous determination of gemfibrozil and rosiglitazone in human plasma.
| العنوان: | Two different spectrofluorimetric methods for simultaneous determination of gemfibrozil and rosiglitazone in human plasma. |
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| المؤلفون: | El-Din MM; Department of Pharmaceutical Analytical Chemistry, College of Pharmacy, Mansoura University, Mansoura, Egypt., Attia KA, Nassar MW, Kaddah MM |
| المصدر: | Talanta [Talanta] 2010 Oct 15; Vol. 82 (5), pp. 1708-16. Date of Electronic Publication: 2010 Aug 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مواضيع طبية MeSH: | Gemfibrozil/*blood , Hypoglycemic Agents/*blood , Spectrometry, Fluorescence/*methods , Thiazolidinediones/*blood, Buffers ; Calibration ; Humans ; Hydrogen-Ion Concentration ; In Vitro Techniques ; Reference Standards ; Reproducibility of Results ; Rosiglitazone ; Sensitivity and Specificity ; Solutions |
| مستخلص: | Two accurate, reliable, and highly sensitive spectrofluorimetric methods were developed for simultaneous determination of binary mixture gemfibrozil and rosiglitazone in human plasma without prior separation steps. The first method is based on synchronous fluorescence spectrometry using double scans. At Δλ=27nm, gemfibrozil yields detectable signal that is independent of the presence of rosiglitazone. Similarly, at Δλ=120nm the signal of rosiglitazone is not influenced by the presence of gemfibrozil. Signals at two wavelengths, 301 (Δλ=27nm) and 368nm (Δλ=120nm) vary linearly with gemfibrozil and rosiglitazone concentrations over the range 100-700ngmL(-1) (for gemfibrozil) and 20-140ngmL(-1) (for rosiglitazone), respectively. The limits of detection (LOD) were 2.3 and 2.72ngmL(-1) for gemfibrozil and rosiglitazone, respectively. The second method is based on the technique of simultaneous equations (Vierodt's method), in which 258nm was selected as the excitation wavelength. Two equations are constructed based on the fact that at ( λ(EM)₂=302 nm of gemfibrozil) and (λ(EM)₂=369 nm of rosiglitazone) the fluorescence of the mixture is the sum of the individual fluorescence of gemfibrozil and rosiglitazone. The limits of detection (LOD) were 28.1 and 23.63ngmL(-1) for gemfibrozil and rosiglitazone, respectively. The proposed methods were successfully applied for the determination of the two compounds in synthetic mixtures and in human plasma with a good recovery. (Copyright © 2010 Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (Buffers) 0 (Hypoglycemic Agents) 0 (Solutions) 0 (Thiazolidinediones) 05V02F2KDG (Rosiglitazone) Q8X02027X3 (Gemfibrozil) |
| تواريخ الأحداث: | Date Created: 20100930 Date Completed: 20110127 Latest Revision: 20181201 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.talanta.2010.07.058 |
| PMID: | 20875567 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2010.07.058 |