دورية أكاديمية
[Isolation, identification, and degrading effect of a pyrene-degrading strain SE12].
| العنوان: | [Isolation, identification, and degrading effect of a pyrene-degrading strain SE12]. |
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| المؤلفون: | Zhang QQ; Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. huijingzi448@163.com, Zhao YJ, Yang CG, Liu FW, He J, Shen B, Ran W |
| المصدر: | Ying yong sheng tai xue bao = The journal of applied ecology [Ying Yong Sheng Tai Xue Bao] 2010 Jul; Vol. 21 (7), pp. 1851-8. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Ying yong sheng tai xue bao bian ji wei yuan hui Country of Publication: China NLM ID: 9425159 Publication Model: Print Cited Medium: Print ISSN: 1001-9332 (Print) Linking ISSN: 10019332 NLM ISO Abbreviation: Ying Yong Sheng Tai Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Shenyang Shi : Ying yong sheng tai xue bao bian ji wei yuan hui Original Publication: Shenyang Shi : Ying yong sheng tai xue bao bian ji wei yuan hui, 1990- |
| مواضيع طبية MeSH: | Biodegradation, Environmental*, Mycobacterium/*isolation & purification , Mycobacterium/*metabolism , Pyrenes/*metabolism , Soil Pollutants/*metabolism, Mycobacterium/genetics ; Soil Microbiology |
| مستخلص: | Using pyrene as the sole carbon and energy source, and by the method of plate sublimation, a strain SE12 was isolated from a contaminated soil near Woniushan Coking Plant in Xuzhou, China. According to the morphological, biochemical, and 16S rDNA analyses, this strain was identified as Mycobacterium sp., with 98% of homology to the rapid-growth nonpathogenic strain M. austroafricanum ATCC 33464. The optimum pH and temperature for the degradation of pyrene by SE12 were pH 9 and 30 degrees C. When the soil samples were added with 100 and 200 mg x kg(-1) of pyrene and inoculated with 10(7) CFU x g(-1) of SE12, the degradation rates of pyrene reached to 97% and 99%, respectively after 28 days incubation at 30 degrees C. By using primer-pairs nidAF/nidAR and nidBF/nidBR for amplification of ring-hydroxylating dioxygenase genes, it was shown that SE12 had the fragments of encoded large and small subunits of dioxygenase genes. Sequence analysis showed that these fragments were highly homologous to the known dioxygenase genes from pyrene-degrading Mycobacteria sp. |
| المشرفين على المادة: | 0 (Pyrenes) 0 (Soil Pollutants) 9E0T7WFW93 (pyrene) |
| تواريخ الأحداث: | Date Created: 20101001 Date Completed: 20110329 Latest Revision: 20161018 |
| رمز التحديث: | 20221213 |
| PMID: | 20879547 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-9332 |
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