التفاصيل البيبلوغرافية
| العنوان: |
Comparison of molecular methods for detection of HPV in oral and oropharyngeal squamous cell carcinoma. |
| المؤلفون: |
Kingma DW; Department of Pathology, Prevention Research Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA., Allen RA, Caughron SK, Melby M, Moore WE, Gillies EM, Marlar RA, Dunn TS |
| المصدر: |
Diagnostic molecular pathology : the American journal of surgical pathology, part B [Diagn Mol Pathol] 2010 Dec; Vol. 19 (4), pp. 218-23. |
| نوع المنشور: |
Comparative Study; Evaluation Study; Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Raven Press Country of Publication: United States NLM ID: 9204924 Publication Model: Print Cited Medium: Internet ISSN: 1533-4066 (Electronic) Linking ISSN: 10529551 NLM ISO Abbreviation: Diagn Mol Pathol Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: New York, N.Y. : Raven Press, c1992- |
| مواضيع طبية MeSH: |
Carcinoma, Squamous Cell/*virology , Molecular Diagnostic Techniques/*methods , Mouth Neoplasms/*virology , Oropharyngeal Neoplasms/*virology , Papillomaviridae/*isolation & purification , Papillomavirus Infections/*diagnosis , Polymerase Chain Reaction/*methods, Humans ; Papillomaviridae/classification |
| مستخلص: |
Substantial molecular evidence exists to implicate human papillomavirus (HPV) in the pathogenesis of a subset of oral and oropharyngeal squamous cell carcinomas. Several studies have shown that HPV-associated oral/oropharyngeal tumors differ etiologically, biologically, and clinically from those that lack the virus. HPV infection confers a significant survival benefit; therefore, HPV detection in tumors could be used to risk-stratify patients and drive optimum treatment strategies. We explored the clinical utility of 6 polymerase chain reaction (PCR)-based or signal amplification-based methods in the detection of HPV in 68 invasive oral/oropharyngeal SSCs and 10 reactive tonsil specimens. Agreement for HPV16 results among the 5 different assays capable of detecting this genotype was substantial (multirater κ=0.72). Only moderate agreement was noted for the 3 assays capable of detecting HPV18 (multirater κ=0.43). HPV results for each assay were evaluated relative to a "majority" HPV result derived from the results of all the detection methods. An HPV16 E6 PCR assay showed the highest concordance with adjudicated consensus HPV16 results (98.7%; κ=0.97), followed by the TaqMan (93.4%; κ=0.87), Linear Array (92.1%; κ=0.84), and E7 PCR (92.1%; κ=0.84) assays, all of which had agreements exceeding 90%, whereas the HPV16/18 Invader assay was lower (85.5%; κ=0.71). The presence of high-risk HPV in a minority of "normal" tonsillar tissues may confound assessment of the virus in oral/oropharyngeal squamous cell carcinoma biopsies using in vitro amplification methods. |
| تواريخ الأحداث: |
Date Created: 20101106 Date Completed: 20110302 Latest Revision: 20191210 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1097/PDM.0b013e3181d0cd35 |
| PMID: |
21051999 |
| قاعدة البيانات: |
MEDLINE |
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