دورية أكاديمية
Novel chimeric thyroid-stimulating hormone-receptor bioassay for thyroid-stimulating immunoglobulins.
العنوان: | Novel chimeric thyroid-stimulating hormone-receptor bioassay for thyroid-stimulating immunoglobulins. |
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المؤلفون: | Lytton SD; Department of Medicine I, Gutenberg University Medical Center, Mainz, Germany., Li Y, Olivo PD, Kohn LD, Kahaly GJ |
المصدر: | Clinical and experimental immunology [Clin Exp Immunol] 2010 Dec; Vol. 162 (3), pp. 438-46. |
نوع المنشور: | Comparative Study; Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0057202 Publication Model: Print Cited Medium: Internet ISSN: 1365-2249 (Electronic) Linking ISSN: 00099104 NLM ISO Abbreviation: Clin Exp Immunol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Blackwell Scientific Publications |
مواضيع طبية MeSH: | Graves Disease/*diagnosis , Receptors, Thyrotropin/*metabolism , Recombinant Fusion Proteins/*metabolism, Animals ; Biological Assay ; CHO Cells ; Cricetinae ; Cricetulus ; Graves Disease/blood ; Graves Disease/immunology ; Graves Disease/physiopathology ; Humans ; Immunoglobulins, Thyroid-Stimulating/blood ; Protein Binding/genetics ; Protein Engineering ; Receptors, Thyrotropin/genetics ; Recombinant Fusion Proteins/genetics ; Reproducibility of Results ; Sensitivity and Specificity ; Transgenes/genetics |
مستخلص: | Thyroid-stimulating immunoglobulins (TSI) are a functional biomarker of Graves' disease (GD). To develop a novel TSI bioassay, a cell line (MC4-CHO-Luc) was bio-engineered to constitutively express a chimeric TSH receptor (TSHR) and constructed with a cyclic adenosine monophosphate (cAMP)-dependent luciferase reporter gene that enables TSI quantification. Data presented as percentage of specimen-to-reference ratio (SRR%) were obtained from 271 patients with various autoimmune and thyroid diseases and 180 controls. Sensitivity of 96% and specificity of 99% for untreated GD were attained by receiver operating characteristic analysis, area under the curve 0·989, 95% confidence interval 0·969-0·999, P = 0·0001. Precision testing of manufactured reagents of high, medium, low and negative SRR% gave a percentage of coefficient-of-variation of 11·5%, 12·8%, 14·5% and 15·7%, respectively. There was no observed interference by haemoglobin, lipids and bilirubin and no non-specific stimulation by various hormones at and above physiological concentrations. TSI levels from GD patients without (SRR% 406 ± 134, mean ± standard deviation) or under anti-thyroid treatment (173 ± 147) were higher (P < 0·0001) compared with TSI levels of patients with Hashimoto's thyroiditis (51 ± 37), autoimmune diseases without GD (24 ± 10), thyroid nodules (30 ± 26) and controls (35 ± 18). The bioassay showed greater sensitivity when compared with anti-TSHR binding assays. In conclusion, the TSI-Mc4 bioassay measures the functional biomarker accurately in GD with a standardized protocol and could improve substantially the diagnosis of autoimmune diseases involving TSHR autoantibodies. (© 2010 The Authors. Clinical and Experimental Immunology © 2010 British Society for Immunology.) |
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المشرفين على المادة: | 0 (Immunoglobulins, Thyroid-Stimulating) 0 (Receptors, Thyrotropin) 0 (Recombinant Fusion Proteins) |
تواريخ الأحداث: | Date Created: 20101113 Date Completed: 20110308 Latest Revision: 20211020 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC3026547 |
DOI: | 10.1111/j.1365-2249.2010.04266.x |
PMID: | 21070207 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1365-2249 |
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DOI: | 10.1111/j.1365-2249.2010.04266.x |