دورية أكاديمية
Differential expression of cellular microRNAs in HPV-11 transfected cells. An analysis by three different array platforms and qRT-PCR.
| العنوان: | Differential expression of cellular microRNAs in HPV-11 transfected cells. An analysis by three different array platforms and qRT-PCR. |
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| المؤلفون: | Dreher A; Institute of Cellular and Molecular Medicine, DNA Tumor Virus Laboratory, University of Copenhagen, Panum Institute, Blegdamsvej 3, DK-2200, Denmark., Rossing M, Kaczkowski B, Nielsen FC, Norrild B |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2010 Dec 17; Vol. 403 (3-4), pp. 357-62. Date of Electronic Publication: 2010 Nov 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Gene Expression Profiling*, Human papillomavirus 11/*genetics , MicroRNAs/*genetics , Papillomavirus Infections/*genetics , Papillomavirus Infections/*virology, Cell Line ; Humans ; Oligonucleotide Array Sequence Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection |
| مستخلص: | Human papillomavirus type 11 (HPV-11) infects the genital and the respiratory tract leading to condylomas and respiratory papillomatosis. HPV infections are restricted to epithelial tissue and the progression through the virus lifecycle is tightly coordinated to the differentiation of the host cell. The changes of cellular microRNAs by HPV-11 gene expression were investigated in a cell culture model of HaCaT cells transfected with HPV-11, with the goal of understanding which cellular processes were affected by the virus. Human microRNA profiling was conducted on three different array platform systems and because very few microRNAs (miR-663, -638, -149* and -92b*) were consistently found in all three array data sets we performed extensive statistical analyses of the array data and the qRT-PCR validation. We assume that the most reliable differentially expressed microRNAs are the ones identified by more than one array platform. We also show that TaqMan® qRT-PCR validation is of limited use for less abundant microRNAs. (Copyright © 2010 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20101117 Date Completed: 20110120 Latest Revision: 20101220 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.bbrc.2010.11.035 |
| PMID: | 21078297 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2010.11.035 |