دورية أكاديمية
Fos-related antigen 1 (Fra-1) pairing with and transactivation of JunB in GBM cells.
| العنوان: | Fos-related antigen 1 (Fra-1) pairing with and transactivation of JunB in GBM cells. |
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| المؤلفون: | Debinski W; Wake Forest University School of Medicine; Winston-Salem NC, USA. debinski@wfubmc.edu, Gibo DM |
| المصدر: | Cancer biology & therapy [Cancer Biol Ther] 2011 Jan 15; Vol. 11 (2), pp. 254-62. Date of Electronic Publication: 2011 Jan 15. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101137842 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1555-8576 (Electronic) Linking ISSN: 15384047 NLM ISO Abbreviation: Cancer Biol Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Georgetown, TX : Landes Bioscience, c2002- |
| مواضيع طبية MeSH: | Brain Neoplasms/*metabolism , Glioblastoma/*metabolism , Proto-Oncogene Proteins c-fos/*genetics , Proto-Oncogene Proteins c-fos/*metabolism , Proto-Oncogene Proteins c-jun/*genetics, Brain Neoplasms/genetics ; Brain Neoplasms/pathology ; Cell Line, Tumor ; Cell Movement/physiology ; Gene Knockdown Techniques ; Gene Silencing ; Glioblastoma/genetics ; Glioblastoma/pathology ; Humans ; Proto-Oncogene Proteins c-jun/metabolism ; RNA, Small Interfering/metabolism ; Trans-Activators/physiology ; Transfection ; Transgenes |
| مستخلص: | Fos-related antigen 1 (Fra-1) plays an important role in maintenance/progression of various cancers, including glioblastoma multiforme (GBM). In this study, we used both shRNA and siRNA to examine the effect of fra-1 knockdown in GBM cells over-expressing Fra-1. Furthermore, we analyzed both the expression of JunB and its knockdown, a previously identified target for Fra-1, and also examined its potential association with Fra-1. When using fra-1 shRNA and siRNA, we found that GBM cells has Fra-1 levels diminished together with the levels of JunB, but Fra-1 remains unchanged in cells with junB knockdown. This is accompanied by dramatic changes in cell morphology and significant alteration in their migration. We next uncovered that the expression of JunB increased in response to ectopic Fra-1 and also to EGF-induced signaling, similarly to Fra-1. This was associated with an avid pairing between phosphorylated Fra-1 and JunB. Importantly, we found that Fra-1 paired with JunB binds to an AP-1 site in the junB gene promoter. JunB knockdown did not affect Fra-1 and the changes in cell morphology did not fully replicate that seen with Fra-1 knockdown. Thus, Fra-1 takes part in a control of architecture and migratory nature of GBM cells. Moreover, Fra-1 is a phosphorylated factor that transactivates JunB with which it makes effectively AP-1 pairs in GBM cells. |
| التعليقات: | Comment in: Cancer Biol Ther. 2011 Feb 1;11(3):307-10. (PMID: 21242721) |
| المشرفين على المادة: | 0 (Proto-Oncogene Proteins c-fos) 0 (Proto-Oncogene Proteins c-jun) 0 (RNA, Small Interfering) 0 (Trans-Activators) 0 (fos-related antigen 1) |
| تواريخ الأحداث: | Date Created: 20101120 Date Completed: 20110426 Latest Revision: 20200930 |
| رمز التحديث: | 20231215 |
| DOI: | 10.4161/cbt.11.2.13953 |
| PMID: | 21088499 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1555-8576 |
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| DOI: | 10.4161/cbt.11.2.13953 |