دورية أكاديمية
H-ras consensus sequence and mutations in primary hepatocellular carcinomas of lemurs and lorises.
| العنوان: | H-ras consensus sequence and mutations in primary hepatocellular carcinomas of lemurs and lorises. |
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| المؤلفون: | Cullen JM; College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. john_cullen@ncsu.edu, Williams C, Zadrozny L, Otstot JT, Solomon GG, Sills RC, Hong HH |
| المصدر: | Veterinary pathology [Vet Pathol] 2011 Jul; Vol. 48 (4), pp. 868-74. Date of Electronic Publication: 2010 Dec 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: United States NLM ID: 0312020 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1544-2217 (Electronic) Linking ISSN: 03009858 NLM ISO Abbreviation: Vet Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2010- : Thousand Oaks, CA : Sage Original Publication: 1971- : Basel : New York : Karger. |
| مواضيع طبية MeSH: | Lemur* , Lorisidae*, Carcinoma, Hepatocellular/*veterinary , Genes, ras/*genetics , Liver Neoplasms/*veterinary , Primate Diseases/*genetics, Animals ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/pathology ; Consensus Sequence ; Female ; Gene Expression Regulation, Neoplastic ; Liver Neoplasms/genetics ; Liver Neoplasms/pathology ; Male ; Mutation ; Primate Diseases/pathology |
| مستخلص: | The authors have determined a consensus sequence for exons 1 and 2 of H-ras from captive lemurs and lorises and evaluated samples of nonneoplastic liver and hepatocellular carcinomas (HCC) from affected animals for mutations in these exons. Frozen liver samples were collected from 20 animals representing 9 different species with a sex distribution of 10 males and 10 females. A total of 26 liver samples, including 11 normal livers, 9 HCC, and 6 samples from nonneoplastic regions of liver from animals with HCC, were evaluated. This is the first report of the consensus sequence for exons 1 and 2 of H-ras in prosimians, and the authors have determined that it is identical to that of human H-ras and differs only slightly from the chimpanzee sequence. Point mutations were identified in 6 of the 9 HCC samples examined with codons 7, 22, 32, 56, 61, 84, and 96 affected. Two carcinomas had double mutations, and one tumor had triple mutations. One HCC had a mutation in codon 61, which is identical to a recognized affected codon for an H-ras "hot spot" in rodent neoplasia that has also been reported in human tumors. Although not statistically different, metastasis occurred in 5 of 6 HCC with H-ras mutation and only 1 of 3 HCC without mutations. There were 4 silent mutations that did not contain changes in the encoded amino acids, 2 of which were found in nonneoplastic regions of tumor-bearing liver. (© The Authors 2011) |
| تواريخ الأحداث: | Date Created: 20101203 Date Completed: 20111128 Latest Revision: 20110701 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1177/0300985810388526 |
| PMID: | 21123858 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1544-2217 |
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| DOI: | 10.1177/0300985810388526 |