دورية أكاديمية
MS analysis of rheumatoid arthritic synovial tissue identifies specific citrullination sites on fibrinogen.
| العنوان: | MS analysis of rheumatoid arthritic synovial tissue identifies specific citrullination sites on fibrinogen. |
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| المؤلفون: | Hermansson M; Department of Medicine, Unit of Rheumatology/Karolinska Biomics Center, Karolinska University Hospital, Karolinska Institute, Stockholm, Sweden., Artemenko K, Ossipova E, Eriksson H, Lengqvist J, Makrygiannakis D, Catrina AI, Nicholas AP, Klareskog L, Savitski M, Zubarev RA, Jakobsson PJ |
| المصدر: | Proteomics. Clinical applications [Proteomics Clin Appl] 2010 May; Vol. 4 (5), pp. 511-8. Date of Electronic Publication: 2010 Jan 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101298608 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1862-8354 (Electronic) Linking ISSN: 18628346 NLM ISO Abbreviation: Proteomics Clin Appl Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : Wiley-VCH, c2007- |
| مواضيع طبية MeSH: | Arthritis, Rheumatoid/*metabolism , Citrulline/*metabolism , Fibrinogen/*metabolism , Synovial Membrane/*metabolism, Aged ; Amino Acid Sequence ; Female ; Fibrinogen/immunology ; Humans ; Male ; Middle Aged ; Protein Processing, Post-Translational ; Spectrometry, Mass, Electrospray Ionization/methods |
| مستخلص: | Purpose: Citrullination is a post-translational modification of arginine residues to citrulline catalyzed by peptidyl arginine deiminases. Induced expression of citrullinated proteins are frequently detected in various inflammatory states including arthritis; however, direct detection of citrullination in arthritic samples has not been successfully performed in the past. Experimental Design: Citrullination of human fibrinogen, a candidate autoantigen in arthritis, was studied. Accurate identification of citrullinated fibrinogen peptides from rheumatoid arthritis synovial tissue specimens was performed using accurate mass and retention time analysis. Results: A peptide with the sequence ESSSHHPGIAEFPSRGK corresponding to amino acids 559-575 of fibrinogen α-chain was identified to be citrullinated with an occupancy rate between 1.4 and 2.5%. Citrullination of the peptide KREEAPSLRPAPPPISGGGYRARPAK corresponding to amino acids 52-77 of the fibrinogen β-chain was identified with an occupancy rate of 1.2%. Conclusions and Clinical Relevance: We report a proof of principle study for the identification of citrullinated proteins and within them, identification of citrullination sites and quantification of their occupancies in synovial tissue from rheumatoid arthritis patients using high-resolution MS. Detailed studies on which molecules are citrullinated in arthritis can provide information about their role in immune regulation and serve as novel biomarkers and potentially even as therapeutic targets. (Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| المشرفين على المادة: | 29VT07BGDA (Citrulline) 9001-32-5 (Fibrinogen) |
| تواريخ الأحداث: | Date Created: 20101208 Date Completed: 20110512 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/prca.200900088 |
| PMID: | 21137068 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1862-8354 |
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| DOI: | 10.1002/prca.200900088 |