دورية أكاديمية
Hypoxia and high glucose activate tetrodotoxin-resistant Na(+) currents through PKA and PKC.
| العنوان: | Hypoxia and high glucose activate tetrodotoxin-resistant Na(+) currents through PKA and PKC. |
|---|---|
| المؤلفون: | Bich-Hoai TT; Department of Anatomy, Physiology and Biophysics, Faculty of Biology, University of Bucharest, Bucharest, Romania., Marin A, Dinu C, Banciu D, Maria-Luiza F, Ristoiu V |
| المصدر: | Acta neurobiologiae experimentalis [Acta Neurobiol Exp (Wars)] 2010; Vol. 70 (4), pp. 351-61. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nencki Institute of Experimental Biology Country of Publication: Poland NLM ID: 1246675 Publication Model: Print Cited Medium: Internet ISSN: 1689-0035 (Electronic) Linking ISSN: 00651400 NLM ISO Abbreviation: Acta Neurobiol Exp (Wars) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Warsaw : Nencki Institute of Experimental Biology Original Publication: Warsaw, Polish Scientific Publishers. |
| مواضيع طبية MeSH: | Cell Hypoxia/*physiology , Cyclic AMP-Dependent Protein Kinases/*metabolism , Glucose/*metabolism , Neurons/*physiology , Protein Kinase C/*metabolism , Sodium Channels/*metabolism, Animals ; Biophysics ; Cell Hypoxia/drug effects ; Cells, Cultured ; Drug Interactions ; Electric Stimulation/methods ; Enzyme Inhibitors/pharmacology ; Ganglia, Spinal/cytology ; Gene Expression Regulation/drug effects ; Male ; Membrane Potentials/drug effects ; Neurons/classification ; Neurons/drug effects ; Patch-Clamp Techniques/methods ; Rats ; Rats, Wistar ; Sodium Channel Blockers/pharmacology ; Sodium Channels/genetics ; Tetrodotoxin/pharmacology |
| مستخلص: | Voltage-gated sodium channels are critical for the initiation and propagation of action potentials and for the regulation of neuronal excitability. Hyperglycemia and hypoxia are two main changes in diabetes frequently associated with several complications. Although many studies on streptozotocin-induced diabetic rats indicate that early diabetic neuropathy is associated with increased amplitude and faster kinetics of sodium channels, the distinctive roles of high glucose and hypoxia have not been completely clarified. Here we show that hypoxic and high glucose conditions (overnight exposure) increase activation and inactivation of TTX-RINa in DRG neurons without affecting the level of expression. Hypoxia and high glucose alone were potent enough to induce similar or even greater sensitization than when both conditions were present, without any of them having a predominant effect. PKA is mainly responsible of the one condition effect, while under both hypoxia and high glucose PKC was also contributing to alter the kinetics, although not in a cumulative manner. These data indicate that TTX-RINa is significantly modulated under short-time exposure to hypoxia and high glucose, a mechanism which might be relevant for diabetes-related complications or other diseases associated with acute hypoxia. |
| المشرفين على المادة: | 0 (Enzyme Inhibitors) 0 (Sodium Channel Blockers) 0 (Sodium Channels) 4368-28-9 (Tetrodotoxin) EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases) EC 2.7.11.13 (Protein Kinase C) IY9XDZ35W2 (Glucose) |
| تواريخ الأحداث: | Date Created: 20110104 Date Completed: 20110503 Latest Revision: 20231207 |
| رمز التحديث: | 20231215 |
| DOI: | 10.55782/ane-2010-1807 |
| PMID: | 21196943 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1689-0035 |
|---|---|
| DOI: | 10.55782/ane-2010-1807 |