دورية أكاديمية
Pathogenicity of gill-associated virus and Mourilyan virus during mixed infections of black tiger shrimp (Penaeus monodon).
| العنوان: | Pathogenicity of gill-associated virus and Mourilyan virus during mixed infections of black tiger shrimp (Penaeus monodon). |
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| المؤلفون: | Oanh DT; CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia., van Hulten MC, Cowley JA, Walker PJ |
| المصدر: | The Journal of general virology [J Gen Virol] 2011 Apr; Vol. 92 (Pt 4), pp. 893-901. Date of Electronic Publication: 2011 Jan 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2099 (Electronic) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology. |
| مواضيع طبية MeSH: | Penaeidae/*virology , Roniviridae/*pathogenicity , Viruses, Unclassified/*pathogenicity, Animal Structures/virology ; Animals ; Australia ; Roniviridae/isolation & purification ; Survival Analysis ; Viral Load ; Viruses, Unclassified/isolation & purification |
| مستخلص: | Gill-associated virus (GAV) and Mourilyan virus (MoV) can occur at very high prevalence in healthy black tiger shrimp (Penaeus monodon) in eastern Australia, and both have been detected in moribund shrimp collected from mid-crop mortality syndrome (MCMS) outbreaks. Experimental evidence presented here indicates that GAV, but not MoV, is the cause of MCMS. Firstly, in healthy P. monodon used for experimental infections, pre-existing MoV genetic loads were very high (mean >10(9) viral RNA copies μg(-1) total RNA) and did not increase significantly following lethal challenge with an inoculum containing both GAV and MoV. In contrast, GAV genetic loads prior to challenge were low (mean ∼10(5) RNA copies μg(-1) total RNA) and increased >10(4)-fold in moribund shrimp. Secondly, dsRNAs targeted to the GAV RNA-dependent RNA polymerase (RdRp) or helicase gene regions reduced GAV genetic loads, delayed the onset of mortalities and improved survival following challenge. In contrast, dsRNA targeted to the MoV RdRp gene (L RNA) was highly effective in reducing MoV genetic loads, but mortality rates were unaffected. Targeting of the MoV S2 RNA, encoding a small non-structural protein (NSs2), a putative supressor of RNA interference, did not reduce the MoV genetic loads or enhance knockdown of GAV when administered simultaneously with dsRNA targeted to the GAV helicase gene. Overall, the data show that P. monodon can tolerate a high-level MoV infection and that mortalities are associated with GAV infection. |
| تواريخ الأحداث: | Date Created: 20110111 Date Completed: 20110518 Latest Revision: 20110317 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1099/vir.0.026724-0 |
| PMID: | 21216987 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2099 |
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| DOI: | 10.1099/vir.0.026724-0 |