دورية أكاديمية
A murine monoclonal anti-factor VIII inhibitory antibody and two human factor VIII inhibitors bind to different areas within a twenty amino acid segment of the acidic region of factor VIII heavy chain.
| العنوان: | A murine monoclonal anti-factor VIII inhibitory antibody and two human factor VIII inhibitors bind to different areas within a twenty amino acid segment of the acidic region of factor VIII heavy chain. |
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| المؤلفون: | Foster PA; Room Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, CA 92037., Fulcher CA, Houghten RA, de Graaf Mahoney S, Zimmerman TS |
| المصدر: | Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis [Blood Coagul Fibrinolysis] 1990 Mar-Apr; Vol. 1 (1), pp. 9-15. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams And Wilkins Country of Publication: England NLM ID: 9102551 Publication Model: Print Cited Medium: Print ISSN: 0957-5235 (Print) Linking ISSN: 09575235 NLM ISO Abbreviation: Blood Coagul Fibrinolysis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Lippincott Williams And Wilkins Original Publication: Oxford, UK : Rapid Communications of Oxford Ltd., c1990- |
| مواضيع طبية MeSH: | Antibodies, Monoclonal/*metabolism , Factor VIII/*metabolism , Peptide Fragments/*metabolism, Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/pharmacology ; Binding Sites ; Factor VIII/antagonists & inhibitors ; Factor VIII/chemistry ; Humans ; Mice ; Molecular Sequence Data ; Peptide Fragments/chemistry ; Peptide Fragments/pharmacology |
| مستخلص: | The factor VIII (FVIII) binding regions of the monoclonal anti-FVIII inhibitory antibody C5 and a human FVIII inhibitor antibody have previously been reported to be contained within amino acid residues 351-365 of FVIII. Localization of the binding regions of these two antibodies was based on their reactivity with four synthetic FVIII peptides. Nineteen synthetic FVIII peptides spanning the entire acidic region of the FVIII heavy chain have now been evaluated for the ability to inhibit the binding of C5 to FVIII in an ELISA assay. The smallest peptide tested that inhibited C5 binding to FVIII consisted of residues 351-361. Those peptides that were able to inhibit C5 binding in the ELISA assay were also able to neutralize the FVIII inhibitory activity of C5 in plasma. The FVIII inhibitory activity of two human FVIII inhibitor antibodies was also partially neutralized by peptides from this region. Evaluation of the pattern of peptides reactive with the three antibodies indicates that the binding regions of these antibodies are in very close proximity to each other, but are not identical. Their respective binding regions are contained within residues 351-361 (C5), 354-362 (inhibitor 1), and 342-354 (inhibitor 2). These results suggest that this 20 amino acid segment of the acidic region of the heavy chain of FVIII may be functionally important in the expression of FVIII procoagulant activity. |
| معلومات مُعتمدة: | DK 07022 United States DK NIDDK NIH HHS; HL 02064 United States HL NHLBI NIH HHS; HL 31950 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Peptide Fragments) 9001-27-8 (Factor VIII) |
| تواريخ الأحداث: | Date Created: 19900301 Date Completed: 19911001 Latest Revision: 20190912 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1097/00001721-199003000-00003 |
| PMID: | 2129392 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0957-5235 |
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| DOI: | 10.1097/00001721-199003000-00003 |